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Anti-WT1 Antibody
Catalog #: 130-10449
Mouse anti-WT1 Antibody (4H8-F11-E12)
Product Description
Specifications
| Size | 20 µg, 100 µg, 200 µg, 500 µg |
| Estimated Lead Time | 1-2 business days |
| Species | Human |
| Accession Number | P19544 |
| Gene Id | 7490 |
| Gene Symbols | WT1 |
| Protein Name / Synonyms | Wilms tumor protein (WT33) |
| Clone | 4H8-F11-E12 |
| Immunogen | Immunogen was recombinant protein. This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with the immunogen. The IgG fraction of tissue culture supernatant was purified by Protein G/A affinity chromatography. |
| Recommended Applications | ELISA (recommended work dilution= 1:4, 000), Western Blotting (recommended work dilution= 1:1, 000-5, 000), |
| Antigen Catalog Number | 230-00676 |
| Reconstitution | Product is supplied as a powder obtained from lyophilization of purified antibody in PBS without preservatives. Reconstitute the antibody with sterile water to a final concentration of 1 mg/ml. |
| Research Area | Cardiovascular Disease, Cancer |
| Shipping Type | Ambient temperature |
| Storage | -20°C |
Description
Description
Wilms tumor protein is a protein that in humans is encoded by the WT1 gene on chromosome 11p.This gene encodes a transcription factor that contains four zinc finger motifs at the C-terminus and a proline / glutamine-rich DNA-binding domain at the N-terminus. It has an essential role in the normal development of the urogenital system, and it is mutated in a subset of patients with Wilms' tumor, the gene's namesake. Multiple transcript variants, resulting from alternative splicing at two coding exons, have been well characterized. There is also evidence for the use of non-AUG (CUG) translation initiation site upstream of, and in-frame with the first AUG, leading to additional isoforms.
Typical Data
Storage/Stability
The antibody is stable for at least 1 year from the date of receipt when stored at -20°C to -70°C. Reconstituted antibody can also be aliquotted and stored at 4°C for 1 month or at -20°C to -70°C in a manual defrost freezer for many months without detectable loss activity. Please avoid freeze-thaw cycles.
Expiration
12 months from the date of shipment when stored properly.