Anti-WT1 (Center E361) Antibody

Product Description

Specifications

Size	400 µL
Estimated Lead Time	1-2 weeks
Species	Human
Host Species	Rabbit
Accession Number	P19544
Gene Id	7490
Gene Symbols	WT1
Protein Name / Synonyms	WT1 (Center E361)
Clonality	Polyclonal
Preparation	This WT1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 346-375 amino acids from the Central region of human WT1.
Concentration (lot specific)	0.300 mg/ml
Recommended Applications	Western Blotting, IHC-P, IF, FC
Research Area	Cardiovascular Disease, Cancer
Shipping Type	Blue ice
Storage	-20°C

Description

Introduction

This gene encodes a transcription factor that contains four zinc-finger motifs at the C-terminus and a proline/glutamine-rich DNA-binding domain at the N-terminus. It has an essential role in the normal development of the urogenital system, and it is mutated in a small subset of patients with Wilm's tumors. This gene exhibits complex tissue-specific and polymorphic imprinting pattern, with biallelic, and monoallelic expression from the maternal and paternal alleles in different tissues. Multiple transcript variants have been described. In several variants, there is evidence for the use of a non-AUG (CUG) translation initiation site upstream of and in-frame with the first AUG. Authors of PMID:7926762 also provide evidence that WT1 mRNA undergoes RNA editing in human and rat, and that this process is tissue-restricted and developmentally regulated. [provided by RefSeq].

Specificity

Predicted Reactivity

Mouse, Rat, Pig, Xenopus

Other Names

WT1; Wilms tumor protein; WT33

NCBI Accession #

NP_000369.3;NP_001185480.1;NP_001185481.1;NP_077742.2;NP_077744.3

Other Accession #

P49952; O62651; P22561; P79958; B7ZSG3; NP_000369

Format

Type

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Calculated Molecular Weight (Da)

49188

Recommended Dilutions

WB: 1:1000; IHC: 1:50-100; IF: 1:10-50; FC: 1:10-50

	WT1 Antibody (Center E361) (Cat# 102-11337) western blot analysis in MCF-7 cell line lysates (35ug/lane).This demonstrates the WT1 antibody detected the WT1 protein (arrow).
	WT1 Antibody (Center E361) (Cat# 102-11337)immunohistochemistry analysis in formalin fixed and paraffin embedded human kidney tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of WT1 Antibody (Center E361) for immunohistochemistry. Clinical relevance has not been evaluated.
	Confocal immunofluorescent analysis of WT1 Antibody (Center E361)(Cat# 102-11337) with MCF-7 cell followed by Alexa Fluor^® 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
	WT1 Antibody (Center E361) (Cat# 102-11337) flow cytometric analysis of MCF-7 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Antigen Source

HUMAN

Storage/Stability

2-8°C (short-term); -20°C (long-term)

Expiration:

12 months from the date of shipment when stored properly.

Featured Products