Anti-XBP1 (Center) Antibody

Product Description

Specifications

Size	400 µL
Estimated Lead Time	1-2 weeks
Species	Human
Host Species	Rabbit
Accession Number	P17861
Gene Id	7494
Gene Symbols	XBP1
Protein Name / Synonyms	XBP1 (Center)
Clonality	Polyclonal
Preparation	This XBP1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 62-91 amino acids from the Central region of human XBP1.
Concentration (lot specific)	0.480 mg/ml
Recommended Applications	Western Blotting, IHC-P, FC
Research Area	Angiogenesis, Autophagy
Shipping Type	Blue ice
Storage	-20°C

Description

Introduction

XBP1 encodes a transcription factor that regulates MHC class II genes by binding to a promoter element referred to as an X box. This gene product is a bZIP protein, which was also identified as a cellular transcription factor that binds to an enhancer in the promoter of the T cell leukemia virus type 1 promoter. It may increase expression of viral proteins by acting as the DNA binding partner of a viral transactivator. It has been found that upon accumulation of unfolded proteins in the endoplasmic reticulum (ER), the mRNA of this gene is processed to an active form by an unconventional splicing mechanism that is mediated by the endonuclease inositol-requiring enzyme 1 (IRE1). The resulting loss of 26 nt from the spliced mRNA causes a frame-shift and an isoform XBP1(S), which is the functionally active transcription factor.

Specificity

Predicted Reactivity

Mouse, Rat, Bovine

Other Names

XBP1; TREB5; XBP2; X-box-binding protein 1; Tax-responsive element-binding protein 5

NCBI Accession #

NP_001073007.1;NP_005071.2

Other Accession #

Q9R1S4; O35426; Q3SZZ2

Format

Type

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Calculated Molecular Weight (Da)

28695

Recommended Dilutions

WB: 1:1000; IHC: 1:50-100; FC: 1:10-50

	Western blot analysis of XBP1 Antibody (Center) (Cat# 102-16402) in Ramos cell line lysates (35ug/lane).XBP1 (arrow) was detected using the purified Pab.
	XBP1 Antibody (Center) (Cat# 102-16402) IHC analysis in formalin fixed and paraffin embedded mouse kidney tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the XBP1 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.
	XBP1 Antibody (Center) (Cat# 102-16402) flow cytometric analysis of Ramos cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
	Western blot analysis of XBP1 (arrow) using rabbit polyclonal XBP1 Antibody (Center) (Cat# 102-16402). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the XBP1 gene.

Antigen Source

HUMAN

Storage/Stability

2-8°C (short-term); -20°C (long-term)

Expiration:

12 months from the date of shipment when stored properly.

Featured Products