Rabbit Anti-Human XRCC1 (Center) Antibody, 400 ul
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Product Description
Specifications
| Size | 400 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human |
| Host Species | Rabbit |
| Accession Number | P18887 |
| Gene Id | 7515 |
| Gene Symbols | XRCC1 |
| Protein Name / Synonyms | XRCC1 (Center) |
| Clonality | Polyclonal |
| Preparation | This XRCC1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 407-435 amino acids from the Central region of human XRCC1. |
| Concentration (lot specific) | 0.5 mg/ml |
| Recommended Applications | Western Blotting, IHC-P, FC, IF |
| Shipping Type | Blue ice |
| Storage | -20°C |
Introduction
The protein encoded by this gene is involved in the efficient repair of DNA single-strand breaks formed by exposure to ionizing radiation and alkylating agents. This protein interacts with DNA ligase III, polymerase beta and poly (ADP-ribose) polymerase to participate in the base excision repair pathway. It may play a role in DNA processing during meiogenesis and recombination in germ cells. A rare microsatellite polymorphism in this gene is associated with cancer in patients of varying radiosensitivity.
Specificity
Other Names
XRCC1; DNA repair protein XRCC1; X-ray repair cross-complementing protein 1
NCBI Accession #
NP_006288.2
Other Accession #
NP_006288.2
Format
Type
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Calculated Molecular Weight (Da)
69477
Recommended Dilutions
IF: 1:25; WB: 1:1000; IHC: 1:50-100; FC: 1:10-50
 | Immunofluorescent analysis of A549 cells, using XRCC1 Antibody (Center) (Cat# 102-16527). 102-16527 was diluted at 1:25 dilution. Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green). Cytoplasmic actin was counterstained with Dylight Fluor® 554 (red) conjugated Phalloidin (red). |
 | XRCC1 Antibody (Center) (Cat# 102-16527) western blot analysis in A375 cell line lysates (15ug/lane).This demonstrates the XRCC1 antibody detected the XRCC1 protein (arrow). |
 | XRCC1 Antibody (Center) (Cat# 102-16527) immunohistochemistry analysis in formalin fixed and paraffin embedded human skin carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the XRCC1 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated. |
 | XRCC1 Antibody (Center) (Cat# 102-16527) flow cytometric analysis of A375 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis. |
Antigen Source
HUMAN
Storage/Stability
2-8°C (short-term); -20°C (long-term)
Expiration:
12 months from the date of shipment when stored properly.