Anti-XRCC4 Antibody

Product Description

Specifications

Size	100 µL
Estimated Lead Time	1-2 weeks
Species	Human
Host Species	Rabbit
Gene Id	7518
Protein Name / Synonyms	XRCC4
Concentration (lot specific)	1 mg/ml
Recommended Applications	ICC/IF, IHC (Formalin-fixed paraffin-embedded sections), Western blot
Shipping Type	Blue ice
Storage	-20°C

Description

Synonyms

XRCC4 antibody, X-ray repair cross-complementing protein 4 antibody, DNA repair protein XRCC4 antibody, X-ray repair, complementing defective, repair in Chinese hamster antibody, X-ray repair complementing defective repair in Chinese hamster cells 4 antibody

Specificity

Species Reactivity

Human

Format

Product Type

Primary antibody

Formulation

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Calculated Molecular Weight

38 kDa

Immunogen

Synthetic peptide corresponding to a region within amino acids 216 and 309 of XRCC4 (Uniprot ID# Q13426)

Applications

Applications Tested

ICC/IF,IHC (Formalin-fixed paraffin-embedded sections),Western blot

Application Notes

Suggested starting dilutions are as follows: WB: 1:500-1:3000, IHC-P: 1:100-1:1000, ICC/IF: 1:100-1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.

XRCC4 antibody detects XRCC4 protein at nucleus on HBL435 xenograft by immunohistochemical analysis.
Sample: Paraffin-embedded HBL435 xenograft.
XRCC4 antibody (107-10858) dilution: 1:500.

Sample (30 ug of whole cell lysate)
A: Jurkat
B: K562
C: HL-60
10% SDS PAGE
107-10858 diluted at 1:1000

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using XRCC4(107-10858) antibody at 1:200 dilution.

Storage

Keep as concentrated solution. Aliquot and store at -20^°C or below. Avoid multiple freeze-thaw cycles.

Expiration:

12 months from the date of shipment when stored properly.

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