Anti-YBX1 (C-term) Antibody

Product Description

Specifications

Size	400 µL
Estimated Lead Time	1-2 weeks
Species	Human
Host Species	Rabbit
Accession Number	P67809
Gene Id	4904
Gene Symbols	YBX1
Protein Name / Synonyms	YBX1 (C-term)
Clonality	Polyclonal
Preparation	This YBX1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 276-305 amino acids from the C-terminal region of human YBX1.
Concentration (lot specific)	2.0 mg/ml
Recommended Applications	Western Blotting, IF
Shipping Type	Blue ice
Storage	-20°C

Description

Introduction

YBX1 binds to splice sites in pre-mRNA and regulates splice site selection. This protein binds and stabilizes cytoplasmic mRNA and contributes to the regulation of translation by modulating the interaction between the mRNA and eukaryotic initiation factors. It binds to promoters that contain a Y-box (5'-CTGATTGGCCAA-3'), such as HLA class II genes. It regulates the transcription of numerous genes and promotes separation of DNA strands that contain mismatches or are modified by cisplatin. It has endonucleolytic activity and can introduce nicks or breaks into double-stranded DNA (in vitro), and it may play a role in DNA repair.

Specificity

Predicted Reactivity

Mouse, Rat, Bovine, Xenopus

Other Names

YBX1; NSEP1; YB1; Nuclease-sensitive element-binding protein 1; CCAAT-binding transcription factor I subunit A; DNA-binding protein B; Enhancer factor I subunit A; Y-box transcription factor; Y-box-binding protein 1

NCBI Accession #

NP_004550.2

Other Accession #

P62961; P62960; P67808; Q00436

Format

Type

Purified Rabbit Polyclonal Antibody (Pab)

Calculated Molecular Weight (Da)

35924

Recommended Dilutions

WB: 1:1000; IF: 1:10-50

	Western blot analysis of YBX1(arrow) using rabbit polyclonal YBX1 Antibody (C-term) (Cat# 102-16735). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the YBX1 gene (Lane 2) (Origene Technologies).
	YBX1 Antibody (C-term) (Cat# 102-16735) western blot analysis in 293 cell line lysates (35ug/lane).This demonstrates the YBX1 antibody detected the YBX1 protein (arrow).
	Fluorescent confocal image of U251 cell stained with YBX1 Antibody (C-term)(Cat# 102-16735).U251 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with YBX1 primary antibody (1:25, 1 h at 37^°). For secondary antibody, Alexa Fluor^® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37^°).Cytoplasmic actin was counterstained with Alexa Fluor^® 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37^°). Nuclei were counterstained with DAPI (blue) (10 µg/ml, 10 min). YBX1 immunoreactivity is localized to Cytoplasm significantly.

Antigen Source

HUMAN

Storage/Stability

2-8°C (short-term); -20°C (long-term)

Expiration:

12 months from the date of shipment when stored properly.

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