Anti-YWHAB (N-term) Antibody

Product Description

Specifications

Size	400 µL
Estimated Lead Time	1-2 weeks
Species	Human
Host Species	Rabbit
Accession Number	P31946
Gene Id	7529
Gene Symbols	YWHAB
Protein Name / Synonyms	YWHAB (N-term)
Clonality	Polyclonal
Preparation	This YWHAB antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 56-85 amino acids from the N-terminal region of human YWHAB.
Concentration (lot specific)	2.0 mg/ml
Recommended Applications	Western Blotting, IHC-P
Shipping Type	Blue ice
Storage	-20°C

Description

Introduction

YWHAB belongs to the 14-3-3 family of proteins, members of which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals. The encoded protein has been shown to interact with RAF1 and CDC25 phosphatases, suggesting that it may play a role in linking mitogenic signaling and the cell cycle machinery. Two transcript variants differing in the 5' UTR, but encoding the same protein, have been identified for the gene. Both variants encode the same protein, however, they are differentially expressed in hematopoietic cells.

Specificity

Predicted Reactivity

Mouse, Rat, Bovine, Monkey

Other Names

YWHAB; 14-3-3 protein beta/alpha; Protein 1054; Protein kinase C inhibitor protein 1; 14-3-3 protein beta/alpha, N-terminally processed

NCBI Accession #

NP_003395.1;NP_647539.1

Other Accession #

P35213; Q9CQV8; Q4R572; P68250

Format

Type

Purified Rabbit Polyclonal Antibody (Pab)

Calculated Molecular Weight (Da)

28082

Recommended Dilutions

WB: 1:1000; IHC: 1:50-100

	The anti-YWHAB Pab (Cat# 102-17837) is used in Western blot to detect YWHAB in HL-60 cell lysate.
	Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
	Western blot analysis of YWHAB (arrow) using rabbit polyclonal YWHAB Antibody (T71) (Cat# 102-17837). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the YWHAB gene.

Antigen Source

HUMAN

Storage/Stability

2-8°C (short-term); -20°C (long-term)

Expiration:

12 months from the date of shipment when stored properly.

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