Anti-YWHAG (Center) Antibody

Product Description

Specifications

Size	400 µL
Estimated Lead Time	1-2 weeks
Species	Human, Mouse
Host Species	Rabbit
Accession Number	P61981
Gene Id	7532
Gene Symbols	YWHAG
Protein Name / Synonyms	YWHAG (Center)
Clonality	Polyclonal
Preparation	This YWHAG antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 136-164 amino acids from the Central region of human YWHAG.
Concentration (lot specific)	2.0 mg/ml
Recommended Applications	Western Blotting, IHC-P, FC
Research Area	Cancer
Shipping Type	Blue ice
Storage	-20°C

Description

Introduction

YWHAG belongs to the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals, and this protein is 100% identical to the rat ortholog. It is induced by growth factors in human vascular smooth muscle cells, and is also highly expressed in skeletal and heart muscles, suggesting an important role for this protein in muscle tissue. It has been shown to interact with RAF1 and protein kinase C, proteins involved in various signal transduction pathways.

Specificity

Predicted Reactivity

Rat, Bovine, Chicken, Xenopus

Other Names

YWHAG; 14-3-3 protein gamma; Protein kinase C inhibitor protein 1; 14-3-3 protein gamma, N-terminally processed

NCBI Accession #

NP_036611.2

Other Accession #

Q6NRY9; Q6PCG0; P61983; P61982; Q5F3W6; P68252

Format

Type

Purified Rabbit Polyclonal Antibody (Pab)

Calculated Molecular Weight (Da)

28303

Recommended Dilutions

WB: 1:1000; IHC: 1:50-100; FC: 1:10-50

	Western blot analysis of lysate from Hela cell line, using YWHAG Antibody (Center)(Cat# 102-15243). 102-15243 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysate at 35ug.
	Western blot analysis of YWHAG Antibody (Center) (Cat# 102-15243) in mouse cerebellum tissue lysates (35ug/lane). YWHAG (arrow) was detected using the purified Pab.
	Formalin-fixed and paraffin-embedded human colon carcinoma reacted with YWHAG Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
	Flow cytometric analysis of widr cells using YWHAG Antibody (Center)(bottom histogram) compared to a negative control cell (top histogram)FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
	Western blot analysis of YWHAG (arrow) using rabbit polyclonal YWHAG Antibody (Center) (Cat# 102-15243). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the YWHAG gene.