Anti-YWHAZ (Center) Antibody

Product Description

Specifications

Size	400 µL
Estimated Lead Time	1-2 weeks
Species	Human, Mouse
Host Species	Rabbit
Accession Number	P63104
Gene Id	7534
Gene Symbols	YWHAZ
Protein Name / Synonyms	YWHAZ (Center)
Clonality	Polyclonal
Preparation	This YWHAZ antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 65-93 amino acids from the Central region of human YWHAZ.
Concentration (lot specific)	0.310 mg/ml
Recommended Applications	Western Blotting, IHC-P, FC
Research Area	Angiogenesis
Shipping Type	Blue ice
Storage	-20°C

Description

Introduction

YWHAZ belongs to the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals, and this protein is 99% identical to the mouse, rat and sheep orthologs. The encoded protein interacts with IRS1 protein, suggesting a role in regulating insulin sensitivity. Two transcript variants differing in the 5' UTR, but encoding the same protein, have been identified for the gene. Both variants encode the same protein, however, they are differentially expressed in hematopoietic cells.

Specificity

Predicted Reactivity

Rat, Bovine, Chicken

Other Names

YWHAZ; 14-3-3 protein zeta/delta; Protein kinase C inhibitor protein 1

NCBI Accession #

NP_001129171.1;NP_001129172.1;NP_001129173.1;NP_001129174.1;NP_003397.1;NP_663723.1

Other Accession #

P63102; P63101; Q5ZKC9; P63103

Format

Type

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Calculated Molecular Weight (Da)

27745

Recommended Dilutions

WB: 1:1000; IHC: 1:50-100; FC: 1:10-50

	Western blot analysis of YWHAZ Antibody (Center) (Cat# 102-17833) in MDA-MB231, A2058 cell line and mouse cerebellum, brain tissue lysates (35ug/lane). YWHAZ (arrow) was detected using the purified Pab.
	YWHAZ Antibody (Center) (Cat# 102-17833) IHC analysis in formalin fixed and paraffin embedded mouse brain followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the YWHAZ Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.
	YWHAZ Antibody (Center) (Cat# 102-17833) flow cytometric analysis of MDA-MB231 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Antigen Source

HUMAN

Storage/Stability

2-8°C (short-term); -20°C (long-term)

Expiration:

12 months from the date of shipment when stored properly.

Featured Products