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Anti-ZAG Antibody, Biotinylated
Catalog #: RB-08-0025B-50
Rabbit anti-ZAG Biotinylated Antibody, 50 ug
Product Description
Specifications
| Size | 50 µg |
| Estimated Lead Time | 1-2 business days |
| Species | Human |
| Host Species | Rabbit |
| Accession Number | P25311 |
| Gene Id | 563 |
| Gene Symbols | AZGP1 ZAG ZNGP1 |
| Clonality | Polyclonal |
| Immunogen | Immunogen was synthetic peptide derived from human Zinc-alpha-2-glycoprotein. This antibody was produced from a rabbit immunized with the immunogen. The IgG fraction was purified from rabbit serum followed by Protein A/G affinity chromatography |
| Immunogen Sequence | CAVPPQDTAPYSCHVQHSS |
| Isotype | IgG |
| Recommended Applications | ELISA (recommended work dilution= 1:2, 000-5, 000), Western Blotting( recommended work dilution= 1:1000-2000), Enzyme Immunoassay (EIA)(recommended work dilution= see related products) |
| Reconstitution | A separate vial of dilution buffer is provided for reconstitution. The antibody is supplied lyophilized, originally containing PBS, without preservative stabilizers (e.g. sodium azide). The final amount is indicated on the shipping vial. |
| Shipping Type | Ambient temperature |
| Storage | -20°C |
Description
Description
Zinc-alpha-2-glycoprotein (ZAG), a 43-kDa protein, is overexpressed in certain human malignant tumors and acts as a lipid-mobilizing factor to stimulate lipolysis in adipocytes leading to cachexia in mice implanted with ZAG-producing tumors. ZAG originally isolated from human plasma, is also found in secretory epithelia cells including those of breast, prostate, and liver, as well as in bronchial, gastrointestinal, and sweat glands. Overexpression of ZAG occurs in several types of malignant tumor, and consequently ZAG has been used as a cancer marker.
Storage / Stability
The antibody is stable for at least 1 year from the date of receipt when stored at -20°C to -70°C. Reconstituted antibody can also be aliquotted and stored at 4°C for 1 month or at -20°C to -70°C in a manual defrost freezer for many months without detectable loss activity. Please avoid freeze-thaw cycles.
Specificity
The antibody can specifically bind to its immunogen, and did not show any cross reactivity with unrelated antigens in ELISA. The specificity for binding to recombinant protein, cellular protein and native antigen is not defined. Cross reactivity with mouse and rat Zinc-alpha-2-glycoprotein was not tested.
Related Products
RayBio® Zinc-binding alpha-2 glycoprotein (ZAG) EIA Kit (cat# EIA-ZAG-1)
References
- Byerley LO, et al. (2010) Evidence for a novel serum factor distinct from zinc alpha-2 glycoprotein that promotes body fat loss early in the development of cachexia. Nutr Cancer. 62(4):484-94.
- Bao Y et al.(2005) Zinc-alpha2-glycoprotein, a lipid mobilizing factor, is expressed and secreted by human (SGBS) adipocytes. FEBS Lett. 579(1):41-7.
Expiration:
12 months from the date of shipment when stored properly.