Anti-ZAP70 (Center) Antibody

Product Description

Specifications

Size	400 µL
Estimated Lead Time	1-2 weeks
Species	Human
Host Species	Rabbit
Accession Number	P43403
Gene Id	7535
Gene Symbols	ZAP70
Protein Name / Synonyms	ZAP70 (Center)
Clonality	Polyclonal
Preparation	This ZAP70 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 276-304 amino acids from the Central region of human ZAP70.
Concentration (lot specific)	0.5 mg/ml
Recommended Applications	Western Blotting, FC, IHC-P
Research Area	Inflammation, Autoimmune Disease
Shipping Type	Blue ice
Storage	-20°C

Description

Introduction

This gene encodes an enzyme belonging to the protein tyrosine kinase family, and it plays a role in T-cell development and lymphocyte activation. This enzyme, which is phosphorylated on tyrosine residues upon T-cell antigen receptor (TCR) stimulation, functions in the initial step of TCR-mediated signal transduction in combination with the Src family kinases, Lck and Fyn. This enzyme is also essential for thymocyte development. Mutations in this gene cause selective T-cell defect, a severe combined immunodeficiency disease characterized by a selective absence of CD8-positive T-cells. Two transcript variants that encode different isoforms have been found for this gene.

Specificity

Other Names

ZAP70; SRK; Tyrosine-protein kinase ZAP-70; 70 kDa zeta-chain associated protein; Syk-related tyrosine kinase

NCBI Accession #

NP_001070.2;NP_997402.1

Other Accession #

NP_001070.2; NP_997402.1

Format

Type

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Calculated Molecular Weight (Da)

69872

Recommended Dilutions

WB: 1:1000; FC: 1:10-50; IHC: 1:10-50

	ZAP70 Antibody (Center) (Cat# 102-12332) western blot analysis in Jurkat cell line lysates (35ug/lane).This demonstrates the ZAP70 antibody detected the ZAP70 protein (arrow).
	ZAP70 Antibody (Center) (Cat# 102-12332) flow cytometric analysis of Ramos cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.
	ZAP70 Antibody (Center) (102-12332)immunohistochemistry analysis in formalin fixed and paraffin embedded human tonsil tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of ZAP70 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.

Antigen Source

HUMAN

Storage/Stability

2-8°C (short-term); -20°C (long-term)

Expiration:

12 months from the date of shipment when stored properly.

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