Anti-ZBTB7B (C-term) Antibody

Product Description

Specifications

Size	400 µL
Estimated Lead Time	1-2 weeks
Species	Human
Host Species	Rabbit
Accession Number	O15156
Gene Id	51043
Gene Symbols	ZBTB7B
Protein Name / Synonyms	ZBTB7B (C-term)
Clonality	Polyclonal
Preparation	This ZBTB7B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 440-469 amino acids from the C-terminal region of human ZBTB7B.
Concentration (lot specific)	2.000 mg/ml
Recommended Applications	Western Blotting, IF
Shipping Type	Blue ice
Storage	-20°C

Description

Introduction

ZBTB7B is a transcription regulator that acts as a key regulator of lineage commitment of immature T-cell precursors. It is necessary and sufficient for commitment of CD4 lineage, while its absence causes CD8 commitment. Development of immature T-cell precursors (thymocytes) to either the CD4 helper or CD8 killer T-cell lineages correlates precisely with their T-cell receptor specificity for major histocompatibility complex class II or class I molecules, respectively. ZBTB7B is a transcriptional repressor of the collagen COL1A1 and COL1A2 genes. It may also function as a repressor of fibronectin and possibly other extracellular matrix genes.

Specificity

Other Names

ZBTB7B; ZBTB15; ZFP67; ZNF857B; Zinc finger and BTB domain-containing protein 7B; Krueppel-related zinc finger protein cKrox; T-helper-inducing POZ/Krueppel-like factor; Zinc finger and BTB domain-containing protein 15; Zinc finger protein 67 homolog; Zinc finger protein 857B; Zinc finger protein Th-POK

NCBI Accession #

NP_001239335.1;NP_001243384.1

Format

Type

Purified Rabbit Polyclonal Antibody (Pab)

Calculated Molecular Weight (Da)

58027

Recommended Dilutions

WB: 1:1000; IF: 1:10-50

Western blot analysis of ZBTB7B Antibody (C-term) (Cat# 102-16762) in 293 cell line lysates (35ug/lane). ZBTB7B (arrow) was detected using the purified Pab.

Fluorescent confocal image of Hela cell stained with ZBTB7B Antibody (C-term)(Cat# 102-16762).Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with ZBTB7B primary antibody (1:25, 1 h at 37^°). For secondary antibody, Alexa Fluor^® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37^°).Cytoplasmic actin was counterstained with Alexa Fluor^® 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37^°). Nuclei were counterstained with DAPI (blue) (10 µg/ml, 10 min). ZBTB7B immunoreactivity is localized to Nucleus significantly.

Antigen Source

HUMAN

Storage/Stability

2-8°C (short-term); -20°C (long-term)

Expiration:

12 months from the date of shipment when stored properly.

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