RayBiotech

3607 Parkway Ln Ste 200 Norcross, Georgia 30092

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ISO 13485:2016 cGMP

Anti-ZHX2 Antibody

Catalog #: 107-11013

ZHX2 antibody [C1C3], 0.86 mg/ml (100 ul)

Product Description

Specifications

Size100 µL
Estimated Lead Time1-2 weeks
SpeciesHuman
Host SpeciesRabbit
Gene Id
22882
Protein Name / Synonyms
ZHX2
Concentration (lot specific)
0.86 mg/ml
Recommended ApplicationsChIP-sequencing, ICC/IF, IHC (Formalin-fixed paraffin-embedded sections), Immunoprecipitation, Western blot
Shipping TypeBlue ice
Storage-20°C

Description

Synonyms

AFR1 antibody, KIAA0854 antibody, RAF antibody, ZHX2 antibody, zinc-fingers and homeoboxes 2 antibody, alpha-fetoprotein regulator 1 antibody, zinc finger and homeodomain protein 2 antibody, transcription factor ZHX2 antibody, zinc fingers and homeoboxes protein 2 antibody, AFP regulator 1 antibody, regulator of AFP antibody, zinc fingers and homeoboxes 2 antibody

Specificity

Species Reactivity
Human

Format

Product Type
Primary antibody
Formulation
0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Calculated Molecular Weight
92 kDa

Immunogen

Recombinant fragment corresponding to a region within amino acids 533 and 826 of ZHX2 (Uniprot ID# Q9Y6X8)

Applications

Applications Tested
ChIP-sequencing,ICC/IF,IHC (Formalin-fixed paraffin-embedded sections),Immunoprecipitation,Western blot
Application Notes
Suggested starting dilutions are as follows: WB: 1:500-1:3000, IHC-P: 1:100-1:1000 [suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)], ICC/IF: 1:100-1:1000, IP [target validated by Mass Spectrometry]. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using ZHX2(107-11013) antibody at 1:200 dilution.
Immunohistochemical analysis of paraffin-embedded SAS Xenograft , using ZHX2(107-11013) antibody at 1:100 dilution.
Sample (30 ug of whole cell lysate)
A: Hep G2
7.5% SDS PAGE
107-11013 diluted at 1:1000

Storage

Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Expiration:
12 months from the date of shipment when stored properly.