Anti-ZHX2 (N-term) Antibody

Product Description

Specifications

Size	400 µL
Estimated Lead Time	1-2 weeks
Species	Human
Host Species	Rabbit
Accession Number	Q9Y6X8
Gene Id	22882
Gene Symbols	ZHX2
Protein Name / Synonyms	ZHX2 (N-term)
Clonality	Polyclonal
Preparation	This ZHX2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 105-131 amino acids from the N-terminal region of human ZHX2.
Concentration (lot specific)	0.5 mg/ml
Recommended Applications	Western Blotting, IF
Shipping Type	Blue ice
Storage	-20°C

Description

Introduction

The members of the zinc fingers and homeoboxes gene family are nuclear homodimeric transcriptional repressors that interact with the A subunit of nuclear factor-Y (NF-YA) and contain two C2H2-type zinc fingers and five homeobox DNA-binding domains. This gene encodes member 2 of this gene family. In addition to forming homodimers, this protein heterodimerizes with member 1 of the zinc fingers and homeoboxes family.

Specificity

Predicted Reactivity

Mouse

Other Names

ZHX2; AFR1; KIAA0854; RAF; Zinc fingers and homeoboxes protein 2; Alpha-fetoprotein regulator 1; Regulator of AFP; Zinc finger and homeodomain protein 2

NCBI Accession #

NP_055758.1

Other Accession #

Q8C0C0; NP_055758.1

Format

Type

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Calculated Molecular Weight (Da)

92307

Recommended Dilutions

WB: 1:1000; IF: 1:10-50

ZHX2 Antibody (N-term)(Cat# 102-13995) western blot analysis in ZR-75-1 cell line lysates (35ug/lane).This demonstrates the ZHX2 antibody detected the ZHX2 protein (arrow).

Fluorescent confocal image of A549 cell stained with ZHX2 Antibody (N-term)(Cat# 102-13995).A549 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with ZHX2 primary antibody (1:25, 1 h at 37^°). For secondary antibody, Alexa Fluor^® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37^°).Cytoplasmic actin was counterstained with Alexa Fluor^® 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37^°). Nuclei were counterstained with DAPI (blue) (10 µg/ml, 10 min). ZHX2 immunoreactivity is localized to Nucleus significantly and Cytoplasm weakly.

Antigen Source

HUMAN

Storage/Stability

2-8°C (short-term); -20°C (long-term)

Expiration:

12 months from the date of shipment when stored properly.

Featured Products