Anti-ZNF143 Antibody

Product Description

Specifications

Size	100 µL
Estimated Lead Time	1-2 weeks
Species	Human
Host Species	Rabbit
Gene Id	7702
Protein Name / Synonyms	ZNF143
Concentration (lot specific)	0.83 mg/ml
Recommended Applications	ICC/IF, Immunoprecipitation, Western blot
Shipping Type	Blue ice
Storage	-20°C

Description

Synonyms

SBF antibody, STAF antibody, pHZ-1 antibody, ZNF143 antibody, selenocysteine tRNA gene transcription-activating factor antibody, SPH-binding factor antibody, hStaf antibody, transcriptional activator Staf antibody, zinc finger protein 143 antibody

Specificity

Species Reactivity

Human

Format

Product Type

Primary antibody

Formulation

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Calculated Molecular Weight

69 kDa

Immunogen

Recombinant fragment corresponding to a region within amino acids 409 and 621 of ZNF143 (Uniprot ID# P52747)

Applications

Applications Tested

ICC/IF,Immunoprecipitation,Western blot

Application Notes

Suggested starting dilutions are as follows: WB: 1:500-1:3000, ICC/IF: 1:100-1:1000, IP: 1:100-1:500. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.

Immunofluorescence analysis of paraformaldehyde-fixed A431, using ZNF143(107-10343) antibody at 1:500 dilution.

ZNF143 antibody immunoprecipitates ZNF143 protein in IP experiments. IP Sample: 293T whole cell lysate/extract A. Control with 2 µg of preimmune rabbit IgG B. Immunoprecipitation of ZNF143 protein by 2 µg of ZNF143 antibody 7.5% SDS-PAGE The immunoprecipitated ZNF143 protein was detected by ZNF143 antibody diluted at 1:1000. EasyBlot anti-rabbit IgG was used as a secondary reagent.

Sample (30 ug of whole cell lysate)
A: Hep G2
7.5% SDS PAGE
107-10343 diluted at 1:1000

Storage

Keep as concentrated solution. Aliquot and store at -20^°C or below. Avoid multiple freeze-thaw cycles.

Expiration:

12 months from the date of shipment when stored properly.

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