Anti-ZNF202 (Center) Antibody

Product Description

Specifications

Size	400 µL
Estimated Lead Time	1-2 weeks
Species	Human
Host Species	Rabbit
Accession Number	O95125
Gene Id	7753
Gene Symbols	ZNF202
Protein Name / Synonyms	ZNF202 (Center)
Clonality	Polyclonal
Preparation	This ZNF202 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 342-370 amino acids from the Central region of human ZNF202.
Concentration (lot specific)	0.480 mg/ml
Recommended Applications	Western Blotting, IHC-P, FC, IF
Shipping Type	Blue ice
Storage	-20°C

Description

Introduction

ZNF202 (Zinc finger protein 202) is a transcriptional repressor of genes affecting the vascular endothelium as well as lipid metabolism and energy homeostasis. Among its targets are structural components of lipoprotein particles (apolipoproteins AIV, CIII, and E), enzymes involved in lipid processing (lipoprotein lipase, lecithin cholesteryl ester transferase), transporters involved in lipid homeostasis (ABCA1, ABCG1), and several genes involved in processes related to energy metabolism and vascular disease.

Specificity

Other Names

ZNF202; ZKSCAN10; Zinc finger protein 202; Zinc finger protein with KRAB and SCAN domains 10

NCBI Accession #

NP_003446.2

Format

Type

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Calculated Molecular Weight (Da)

74720

Recommended Dilutions

WB: 1:1000; IHC: 1:50-100; FC: 1:10-50; IF: 1:10-50

	Western blot analysis of ZNF202 Antibody (Center) (Cat# 102-18388) in HepG2 cell line lysates (35ug/lane). ZNF202 (arrow) was detected using the purified Pab.
	ZNF202 Antibody (Center) (Cat# 102-18388) IHC analysis in formalin fixed and paraffin embedded breast carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the ZNF202 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.
	ZNF202 Antibody (Center) (Cat# 102-18388) flow cytometric analysis of HepG2 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
	Confocal immunofluorescent analysis of ZNF202 Antibody (Center) (Cat# 102-18388) with HepG2 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
	Fluorescent confocal image of MDA-MB231 cell stained with ZNF202 Antibody (Center)(Cat# 102-18388). MDA-MB231 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with ZNF202 primary antibody (1:25, 1 h at 37^°). For secondary antibody, Alexa Fluor^® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37^°).Cytoplasmic actin was counterstained with Alexa Fluor^® 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37^°). Nuclei were counterstained with DAPI (blue) (10 µg/ml, 10 min).ZNF202 immunoreactivity is localized to nucleus significantly.