Anti-ZNF259 Antibody

Product Description

Specifications

Size	100 µL
Estimated Lead Time	1-2 weeks
Species	Human
Host Species	Rabbit
Gene Id	8882
Protein Name / Synonyms	ZNF259
Concentration (lot specific)	0.96 mg/ml
Recommended Applications	ICC/IF, IHC (Formalin-fixed paraffin-embedded sections), Western blot
Shipping Type	Blue ice
Storage	-20°C

Description

Synonyms

MGC110983 antibody, ZPR1 antibody, ZNF259 antibody, zinc finger protein ZPR1 antibody, zinc finger protein 259 antibody

Specificity

Species Reactivity

Human

Format

Product Type

Primary antibody

Formulation

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Calculated Molecular Weight

51 kDa

Immunogen

Recombinant fragment corresponding to a region within amino acids 159 and 459 of ZNF259 (Uniprot ID# O75312)

Applications

Applications Tested

ICC/IF,IHC (Formalin-fixed paraffin-embedded sections),Western blot

Application Notes

Suggested starting dilutions are as follows: WB: 1:500-1:3000, IHC-P: 1:100-1:1000 [suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)], ICC/IF: 1:100-1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.

Immunofluorescence analysis of paraformaldehyde-fixed A431, using ZNF259(107-10836) antibody at 1:200 dilution.

Immunohistochemical analysis of paraffin-embedded NCIN87 xenograft, using ZNF259(107-10836) antibody at 1:500 dilution.

Sample (30 ug of whole cell lysate)
A: A431
10% SDS PAGE
107-10836 diluted at 1:1000

Storage

Keep as concentrated solution. Aliquot and store at -20^°C or below. Avoid multiple freeze-thaw cycles.

Expiration:

12 months from the date of shipment when stored properly.

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