Anti-ZO-1 (C-term) Antibody

Product Description

Specifications

Size	100 µL
Estimated Lead Time	1-2 weeks
Species	Human
Host Species	Rabbit
Gene Id	7082
Protein Name / Synonyms	ZO-1, C-term
Concentration (lot specific)	1 mg/ml
Recommended Applications	Western blot
Shipping Type	Blue ice
Storage	-20°C

Description

Synonyms

DKFZp686M05161 antibody, MGC133289 antibody, ZO-1 antibody, TJP1 antibody, zona occludens protein 1 antibody, zona occludens 1 antibody, tight junction protein ZO-1 antibody, zonula occludens 1 protein antibody, zonula occludens protein 1 antibody, tight junction protein 1 (zona occludens 1) antibody

Specificity

Species Reactivity

Human

Format

Product Type

Primary antibody

Formulation

1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Calculated Molecular Weight

195 kDa

Immunogen

Recombinant fragment corresponding to a region within amino acids 1574 and 1748 of ZO-1 (Uniprot ID# Q07157)

Applications

Applications Tested

Western blot

Application Notes

Suggested starting dilutions are as follows: WB: 1:500-1:3000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.

Sample (30 ug of whole cell lysate)
A: HCT116
5% SDS PAGE
107-10779 diluted at 1:1000

ZO-1 antibody detects ZO-1 protein by western blot analysis.
A. 30 µg 293T whole cell lysate/extract
B. 30 µg A431 whole cell lysate/extract
C. 30 µg HeLa whole cell lysate/extract
5 % SDS-PAGE
ZO-1 antibody (107-10779) dilution: 1:500

Storage

Keep as concentrated solution. Aliquot and store at -20^°C or below. Avoid multiple freeze-thaw cycles.

Expiration:

12 months from the date of shipment when stored properly.

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