Epitope Mapping Service

How It Works

RayBiotech’s peptide-based epitope mapping arrays are prepared by first printing overlapping, chemically-synthesized peptides (> 95% purity) spanning the length of the protein-of-interest onto a glass slide surface in an arrayed format, with each peptide spotted in triplicate. Multiple positive and negative controls are also printed onto the array to monitor each incubation step. Purified antibodies or bodily fluids containing antibodies are then diluted and incubated on the peptide arrays, during which the antibodies specifically bind to the peptides displaying their sequence-of-interest. After unbound antibodies are washed off the array, a biotin-conjugated secondary antibody (e.g., anti-human IgG, mouse IgG) and a fluorescence dye-conjugated streptavidin molecule are added to the array to enable the detection of bound antibodies bound. Fluorescent signal is then captured using a laser scanner. Since the peptide sequence of each spot is known, the antibody’s potential epitope(s) can be ascertained.

In addition to custom epitope mapping arrays, RayBiotech offers pre-designed peptide panels for popular drug targets. For more information, please contact our technical support team at techsupport@raybiotech.com.