High Density

TNF alpha Epitope Mapping Peptide Array

The TNF alpha Epitope Mapping Peptide Array is a semi-quantitative array displaying 222 synthesized peptides representing the full-length TNFA. It is suitable for antibody epitope mapping and detection of IgG, IgA, IgM, IgE, etc. in serum, plasma, and many other liquid sample types.

For a service quote, please fill out the Epitope Mapping Array Quote Request Form.

Product Description

Specifications

Size	4 Sample Kit, 8 Sample Kit, 16 Sample Kit
Estimated Lead Time	4-6 weeks
Species	Human
Compatible Sample Types	Plasma, Serum, Ascites, Hybridoma cell culture medium, Purified Antibodies, Purified Proteins, DNA Aptamers
Accession Number	P01375
Gene Symbols	TNFA, TNFSF2
Protein Name / Synonyms	Tumor necrosis factor (Cachectin) (TNF-alpha) (Tumor necrosis factor ligand superfamily member 2) (TNF-a) [Cleaved into: Tumor necrosis factor, membrane form (N-terminal fragment) (NTF) Intracellular domain 1 (ICD1) Intracellular domain 2 (ICD2) C-domain 1 C-domain 2 Tumor necrosis factor, soluble form]
Quantitative/Semi-Quantitative	Semi-Quantitative
Number of Targets Detected	222
Solid Support	Glass Slide
Method Of Detection	Fluorescence Laser Scanner
Research Area	Inflammation, Antibody Drug Development, Drug Antibody (DA) Detection, Allergy
Shipping Type	Blue ice
Storage	-20°C

Peptide Information

Number of Peptides:

222

Peptide Length:

12-mer

Peptide Offset:

1-mer

Peptide Purity:

HPLC grade, >95% purity

Array Printing Replicate:

Triplicate

Peptide Sequence

1. MSTESMIRDVEL	2. STESMIRDVELA	3. TESMIRDVELAE	4. ESMIRDVELAEE
5. SMIRDVELAEEA	6. MIRDVELAEEAL	7. IRDVELAEEALP	8. RDVELAEEALPK
9. DVELAEEALPKK	10. VELAEEALPKKT	11. ELAEEALPKKTG	12. LAEEALPKKTGG
13. AEEALPKKTGGP	14. EEALPKKTGGPQ	15. EALPKKTGGPQG	16. ALPKKTGGPQGS
17. LPKKTGGPQGSR	18. PKKTGGPQGSRR	19. KKTGGPQGSRRC	20. KTGGPQGSRRCL
21. TGGPQGSRRCLF	22. GGPQGSRRCLFL	23. GPQGSRRCLFLS	24. PQGSRRCLFLSL
25. QGSRRCLFLSLF	26. GSRRCLFLSLFS	27. SRRCLFLSLFSF	28. RRCLFLSLFSFL
29. RCLFLSLFSFLI	30. CLFLSLFSFLIV	31. LFLSLFSFLIVA	32. FLSLFSFLIVAG
33. LSLFSFLIVAGA	34. SLFSFLIVAGAT	35. LFSFLIVAGATT	36. FSFLIVAGATTL
37. SFLIVAGATTLF	38. FLIVAGATTLFC	39. LIVAGATTLFCL	40. IVAGATTLFCLL
41. VAGATTLFCLLH	42. AGATTLFCLLHF	43. GATTLFCLLHFG	44. ATTLFCLLHFGV
45. TTLFCLLHFGVI	46. TLFCLLHFGVIG	47. LFCLLHFGVIGP	48. FCLLHFGVIGPQ
49. CLLHFGVIGPQR	50. LLHFGVIGPQRE	51. LHFGVIGPQREE	52. HFGVIGPQREEF
53. FGVIGPQREEFP	54. GVIGPQREEFPR	55. VIGPQREEFPRD	56. IGPQREEFPRDL
57. GPQREEFPRDLS	58. PQREEFPRDLSL	59. QREEFPRDLSLI	60. REEFPRDLSLIS
61. EEFPRDLSLISP	62. EFPRDLSLISPL	63. FPRDLSLISPLA	64. PRDLSLISPLAQ
65. RDLSLISPLAQA	66. DLSLISPLAQAV	67. LSLISPLAQAVR	68. SLISPLAQAVRS
69. LISPLAQAVRSS	70. ISPLAQAVRSSS	71. SPLAQAVRSSSR	72. PLAQAVRSSSRT
73. LAQAVRSSSRTP	74. AQAVRSSSRTPS	75. QAVRSSSRTPSD	76. AVRSSSRTPSDK
77. VRSSSRTPSDKP	78. RSSSRTPSDKPV	79. SSSRTPSDKPVA	80. SSRTPSDKPVAH
81. SRTPSDKPVAHV	82. RTPSDKPVAHVV	83. TPSDKPVAHVVA	84. PSDKPVAHVVAN
85. SDKPVAHVVANP	86. DKPVAHVVANPQ	87. KPVAHVVANPQA	88. PVAHVVANPQAE
89. VAHVVANPQAEG	90. AHVVANPQAEGQ	91. HVVANPQAEGQL	92. VVANPQAEGQLQ
93. VANPQAEGQLQW	94. ANPQAEGQLQWL	95. NPQAEGQLQWLN	96. PQAEGQLQWLNR
97. QAEGQLQWLNRR	98. AEGQLQWLNRRA	99. EGQLQWLNRRAN	100. GQLQWLNRRANA
101. QLQWLNRRANAL	102. LQWLNRRANALL	103. QWLNRRANALLA	104. WLNRRANALLAN
105. LNRRANALLANG	106. NRRANALLANGV	107. RRANALLANGVE	108. RANALLANGVEL
109. ANALLANGVELR	110. NALLANGVELRD	111. ALLANGVELRDN	112. LLANGVELRDNQ
113. LANGVELRDNQL	114. ANGVELRDNQLV	115. NGVELRDNQLVV	116. GVELRDNQLVVP
117. VELRDNQLVVPS	118. ELRDNQLVVPSE	119. LRDNQLVVPSEG	120. RDNQLVVPSEGL
121. DNQLVVPSEGLY	122. NQLVVPSEGLYL	123. QLVVPSEGLYLI	124. LVVPSEGLYLIY
125. VVPSEGLYLIYS	126. VPSEGLYLIYSQ	127. PSEGLYLIYSQV	128. SEGLYLIYSQVL
129. EGLYLIYSQVLF	130. GLYLIYSQVLFK	131. LYLIYSQVLFKG	132. YLIYSQVLFKGQ
133. LIYSQVLFKGQG	134. IYSQVLFKGQGC	135. YSQVLFKGQGCP	136. SQVLFKGQGCPS
137. QVLFKGQGCPST	138. VLFKGQGCPSTH	139. LFKGQGCPSTHV	140. FKGQGCPSTHVL
141. KGQGCPSTHVLL	142. GQGCPSTHVLLT	143. QGCPSTHVLLTH	144. GCPSTHVLLTHT
145. CPSTHVLLTHTI	146. PSTHVLLTHTIS	147. STHVLLTHTISR	148. THVLLTHTISRI
149. HVLLTHTISRIA	150. VLLTHTISRIAV	151. LLTHTISRIAVS	152. LTHTISRIAVSY
153. THTISRIAVSYQ	154. HTISRIAVSYQT	155. TISRIAVSYQTK	156. ISRIAVSYQTKV
157. SRIAVSYQTKVN	158. RIAVSYQTKVNL	159. IAVSYQTKVNLL	160. AVSYQTKVNLLS
161. VSYQTKVNLLSA	162. SYQTKVNLLSAI	163. YQTKVNLLSAIK	164. QTKVNLLSAIKS
165. TKVNLLSAIKSP	166. KVNLLSAIKSPC	167. VNLLSAIKSPCQ	168. NLLSAIKSPCQR
169. LLSAIKSPCQRE	170. LSAIKSPCQRET	171. SAIKSPCQRETP	172. AIKSPCQRETPE
173. IKSPCQRETPEG	174. KSPCQRETPEGA	175. SPCQRETPEGAE	176. PCQRETPEGAEA
177. CQRETPEGAEAK	178. QRETPEGAEAKP	179. RETPEGAEAKPW	180. ETPEGAEAKPWY
181. TPEGAEAKPWYE	182. PEGAEAKPWYEP	183. EGAEAKPWYEPI	184. GAEAKPWYEPIY
185. AEAKPWYEPIYL	186. EAKPWYEPIYLG	187. AKPWYEPIYLGG	188. KPWYEPIYLGGV
189. PWYEPIYLGGVF	190. WYEPIYLGGVFQ	191. YEPIYLGGVFQL	192. EPIYLGGVFQLE
193. PIYLGGVFQLEK	194. IYLGGVFQLEKG	195. YLGGVFQLEKGD	196. LGGVFQLEKGDR
197. GGVFQLEKGDRL	198. GVFQLEKGDRLS	199. VFQLEKGDRLSA	200. FQLEKGDRLSAE
201. QLEKGDRLSAEI	202. LEKGDRLSAEIN	203. EKGDRLSAEINR	204. KGDRLSAEINRP
205. GDRLSAEINRPD	206. DRLSAEINRPDY	207. RLSAEINRPDYL	208. LSAEINRPDYLD
209. SAEINRPDYLDF	210. AEINRPDYLDFA	211. EINRPDYLDFAE	212. INRPDYLDFAES
213. NRPDYLDFAESG	214. RPDYLDFAESGQ	215. PDYLDFAESGQV	216. DYLDFAESGQVY
217. YLDFAESGQVYF	218. LDFAESGQVYFG	219. DFAESGQVYFGI	220. FAESGQVYFGII
221. AESGQVYFGIIA	222. ESGQVYFGIIAL

Application Notes

Identify high antigenicity epitopes for antibody production & vaccine development
Locate (auto)antigen epitopes of (auto)antibodies
Define the best antibody clone against the protein-of-interest
Perform epitope mutant analysis
Map B cell epitopes

Service Features

Small serum sample volume required (2 µl)
High density (simultaneously analyze hundreds of overlapping peptides)
High sensitivity
Large dynamic range
Suitable for high-throughput assays
High efficiency and accuracy
Affordable, quick and simple to use

Kit Components

RayBio^® human TNF alpha protein peptide array
Blocking Buffer
Wash Buffer I
Wash Buffer II
Biotin-conjugated secondary antibody
Cy3 equivalent dye conjugated streptavidin
Array accessories (including slide incubation chamber assembly, gasket, adhesive plastic strips, etc.)

Other Materials Required

Distilled water
Small plastic boxes or containers
Orbital shaker or oscillating rocker
Pipettors, pipette tips and other common lab consumables
Aluminum foil
Laser fluorescence scanner

View Compatible Laser Scanners

Don't have a compatible scanner? RayBiotech now offers FREE scanning service for all RayBio glass slide antibody arrays! Learn More

Storage/Stability

Upon receipt, all kit components should be stored at -20 °C to -80 °C until ready to use. Stored properly, the kit will retain complete activity for up to 6 months.

Peptide Information

Number of Peptides:

222

Peptide Length:

12-mer

Peptide Offset:

1-mer

Peptide Purity:

HPLC grade, >95% purity

Array Printing Replicate:

Triplicate

Peptide Sequence

1. MSTESMIRDVEL	2. STESMIRDVELA	3. TESMIRDVELAE	4. ESMIRDVELAEE
5. SMIRDVELAEEA	6. MIRDVELAEEAL	7. IRDVELAEEALP	8. RDVELAEEALPK
9. DVELAEEALPKK	10. VELAEEALPKKT	11. ELAEEALPKKTG	12. LAEEALPKKTGG
13. AEEALPKKTGGP	14. EEALPKKTGGPQ	15. EALPKKTGGPQG	16. ALPKKTGGPQGS
17. LPKKTGGPQGSR	18. PKKTGGPQGSRR	19. KKTGGPQGSRRC	20. KTGGPQGSRRCL
21. TGGPQGSRRCLF	22. GGPQGSRRCLFL	23. GPQGSRRCLFLS	24. PQGSRRCLFLSL
25. QGSRRCLFLSLF	26. GSRRCLFLSLFS	27. SRRCLFLSLFSF	28. RRCLFLSLFSFL
29. RCLFLSLFSFLI	30. CLFLSLFSFLIV	31. LFLSLFSFLIVA	32. FLSLFSFLIVAG
33. LSLFSFLIVAGA	34. SLFSFLIVAGAT	35. LFSFLIVAGATT	36. FSFLIVAGATTL
37. SFLIVAGATTLF	38. FLIVAGATTLFC	39. LIVAGATTLFCL	40. IVAGATTLFCLL
41. VAGATTLFCLLH	42. AGATTLFCLLHF	43. GATTLFCLLHFG	44. ATTLFCLLHFGV
45. TTLFCLLHFGVI	46. TLFCLLHFGVIG	47. LFCLLHFGVIGP	48. FCLLHFGVIGPQ
49. CLLHFGVIGPQR	50. LLHFGVIGPQRE	51. LHFGVIGPQREE	52. HFGVIGPQREEF
53. FGVIGPQREEFP	54. GVIGPQREEFPR	55. VIGPQREEFPRD	56. IGPQREEFPRDL
57. GPQREEFPRDLS	58. PQREEFPRDLSL	59. QREEFPRDLSLI	60. REEFPRDLSLIS
61. EEFPRDLSLISP	62. EFPRDLSLISPL	63. FPRDLSLISPLA	64. PRDLSLISPLAQ
65. RDLSLISPLAQA	66. DLSLISPLAQAV	67. LSLISPLAQAVR	68. SLISPLAQAVRS
69. LISPLAQAVRSS	70. ISPLAQAVRSSS	71. SPLAQAVRSSSR	72. PLAQAVRSSSRT
73. LAQAVRSSSRTP	74. AQAVRSSSRTPS	75. QAVRSSSRTPSD	76. AVRSSSRTPSDK
77. VRSSSRTPSDKP	78. RSSSRTPSDKPV	79. SSSRTPSDKPVA	80. SSRTPSDKPVAH
81. SRTPSDKPVAHV	82. RTPSDKPVAHVV	83. TPSDKPVAHVVA	84. PSDKPVAHVVAN
85. SDKPVAHVVANP	86. DKPVAHVVANPQ	87. KPVAHVVANPQA	88. PVAHVVANPQAE
89. VAHVVANPQAEG	90. AHVVANPQAEGQ	91. HVVANPQAEGQL	92. VVANPQAEGQLQ
93. VANPQAEGQLQW	94. ANPQAEGQLQWL	95. NPQAEGQLQWLN	96. PQAEGQLQWLNR
97. QAEGQLQWLNRR	98. AEGQLQWLNRRA	99. EGQLQWLNRRAN	100. GQLQWLNRRANA
101. QLQWLNRRANAL	102. LQWLNRRANALL	103. QWLNRRANALLA	104. WLNRRANALLAN
105. LNRRANALLANG	106. NRRANALLANGV	107. RRANALLANGVE	108. RANALLANGVEL
109. ANALLANGVELR	110. NALLANGVELRD	111. ALLANGVELRDN	112. LLANGVELRDNQ
113. LANGVELRDNQL	114. ANGVELRDNQLV	115. NGVELRDNQLVV	116. GVELRDNQLVVP
117. VELRDNQLVVPS	118. ELRDNQLVVPSE	119. LRDNQLVVPSEG	120. RDNQLVVPSEGL
121. DNQLVVPSEGLY	122. NQLVVPSEGLYL	123. QLVVPSEGLYLI	124. LVVPSEGLYLIY
125. VVPSEGLYLIYS	126. VPSEGLYLIYSQ	127. PSEGLYLIYSQV	128. SEGLYLIYSQVL
129. EGLYLIYSQVLF	130. GLYLIYSQVLFK	131. LYLIYSQVLFKG	132. YLIYSQVLFKGQ
133. LIYSQVLFKGQG	134. IYSQVLFKGQGC	135. YSQVLFKGQGCP	136. SQVLFKGQGCPS
137. QVLFKGQGCPST	138. VLFKGQGCPSTH	139. LFKGQGCPSTHV	140. FKGQGCPSTHVL
141. KGQGCPSTHVLL	142. GQGCPSTHVLLT	143. QGCPSTHVLLTH	144. GCPSTHVLLTHT
145. CPSTHVLLTHTI	146. PSTHVLLTHTIS	147. STHVLLTHTISR	148. THVLLTHTISRI
149. HVLLTHTISRIA	150. VLLTHTISRIAV	151. LLTHTISRIAVS	152. LTHTISRIAVSY
153. THTISRIAVSYQ	154. HTISRIAVSYQT	155. TISRIAVSYQTK	156. ISRIAVSYQTKV
157. SRIAVSYQTKVN	158. RIAVSYQTKVNL	159. IAVSYQTKVNLL	160. AVSYQTKVNLLS
161. VSYQTKVNLLSA	162. SYQTKVNLLSAI	163. YQTKVNLLSAIK	164. QTKVNLLSAIKS
165. TKVNLLSAIKSP	166. KVNLLSAIKSPC	167. VNLLSAIKSPCQ	168. NLLSAIKSPCQR
169. LLSAIKSPCQRE	170. LSAIKSPCQRET	171. SAIKSPCQRETP	172. AIKSPCQRETPE
173. IKSPCQRETPEG	174. KSPCQRETPEGA	175. SPCQRETPEGAE	176. PCQRETPEGAEA
177. CQRETPEGAEAK	178. QRETPEGAEAKP	179. RETPEGAEAKPW	180. ETPEGAEAKPWY
181. TPEGAEAKPWYE	182. PEGAEAKPWYEP	183. EGAEAKPWYEPI	184. GAEAKPWYEPIY
185. AEAKPWYEPIYL	186. EAKPWYEPIYLG	187. AKPWYEPIYLGG	188. KPWYEPIYLGGV
189. PWYEPIYLGGVF	190. WYEPIYLGGVFQ	191. YEPIYLGGVFQL	192. EPIYLGGVFQLE
193. PIYLGGVFQLEK	194. IYLGGVFQLEKG	195. YLGGVFQLEKGD	196. LGGVFQLEKGDR
197. GGVFQLEKGDRL	198. GVFQLEKGDRLS	199. VFQLEKGDRLSA	200. FQLEKGDRLSAE
201. QLEKGDRLSAEI	202. LEKGDRLSAEIN	203. EKGDRLSAEINR	204. KGDRLSAEINRP
205. GDRLSAEINRPD	206. DRLSAEINRPDY	207. RLSAEINRPDYL	208. LSAEINRPDYLD
209. SAEINRPDYLDF	210. AEINRPDYLDFA	211. EINRPDYLDFAE	212. INRPDYLDFAES
213. NRPDYLDFAESG	214. RPDYLDFAESGQ	215. PDYLDFAESGQV	216. DYLDFAESGQVY
217. YLDFAESGQVYF	218. LDFAESGQVYFG	219. DFAESGQVYFGI	220. FAESGQVYFGII
221. AESGQVYFGIIA	222. ESGQVYFGIIAL

Application Notes

Identify high antigenicity epitopes for antibody production & vaccine development
Locate (auto)antigen epitopes of (auto)antibodies
Define the best antibody clone against the protein-of-interest
Perform epitope mutant analysis
Map B cell epitopes

Service Features

Small serum sample volume required (2 µl)
High density (simultaneously analyze hundreds of overlapping peptides)
High sensitivity
Large dynamic range
Suitable for high-throughput assays
High efficiency and accuracy
Affordable, quick and simple to use

Kit Components

RayBio^® human TNF alpha protein peptide array
Blocking Buffer
Wash Buffer I
Wash Buffer II
Biotin-conjugated secondary antibody
Cy3 equivalent dye conjugated streptavidin
Array accessories (including slide incubation chamber assembly, gasket, adhesive plastic strips, etc.)

Other Materials Required

Distilled water
Small plastic boxes or containers
Orbital shaker or oscillating rocker
Pipettors, pipette tips and other common lab consumables
Aluminum foil
Laser fluorescence scanner

View Compatible Laser Scanners

Don't have a compatible scanner? RayBiotech now offers FREE scanning service for all RayBio glass slide antibody arrays! Learn More

Storage/Stability

Upon receipt, all kit components should be stored at -20 °C to -80 °C until ready to use. Stored properly, the kit will retain complete activity for up to 6 months.

Featured Products

TNF alpha Epitope Mapping Peptide Array

Product Description

Specifications

Peptide Information

Peptide Sequence

Application Notes

Service Features

Kit Components

Other Materials Required

Storage/Stability

Featured Products

TNF alpha Epitope Mapping Peptide Array

Product Description

Specifications

Peptide Information

Peptide Sequence

Application Notes

Service Features

Kit Components

Other Materials Required

Storage/Stability

TNF alpha Epitope Mapping Peptide Array

TNF alpha Epitope Mapping Peptide Array

Featured Products

TNF alpha Epitope Mapping Peptide Array

Product Description

Specifications

Peptide Information

Peptide Sequence

Application Notes

Service Features

Kit Components

Other Materials Required

Storage/Stability

Reviews

Ask A Question

Featured Products

TNF alpha Epitope Mapping Peptide Array

Product Description

Specifications

Peptide Information

Peptide Sequence

Application Notes

Service Features

Kit Components

Other Materials Required

Storage/Stability

Reviews

Ask A Question

TNF alpha Epitope Mapping Peptide Array

TNF alpha Epitope Mapping Peptide Array