1. FTVTVPKDLYVV	2. TVTVPKDLYVVE	3. VTVPKDLYVVEY	4. TVPKDLYVVEYG
5. VPKDLYVVEYGS	6. PKDLYVVEYGSN	7. KDLYVVEYGSNM	8. DLYVVEYGSNMT
9. LYVVEYGSNMTI	10. YVVEYGSNMTIE	11. VVEYGSNMTIEC	12. VEYGSNMTIECK
13. EYGSNMTIECKF	14. YGSNMTIECKFP	15. GSNMTIECKFPV	16. SNMTIECKFPVE
17. NMTIECKFPVEK	18. MTIECKFPVEKQ	19. TIECKFPVEKQL	20. IECKFPVEKQLD
21. ECKFPVEKQLDL	22. CKFPVEKQLDLA	23. KFPVEKQLDLAA	24. FPVEKQLDLAAL
25. PVEKQLDLAALI	26. VEKQLDLAALIV	27. EKQLDLAALIVY	28. KQLDLAALIVYW
29. QLDLAALIVYWE	30. LDLAALIVYWEM	31. DLAALIVYWEME	32. LAALIVYWEMED
33. AALIVYWEMEDK	34. ALIVYWEMEDKN	35. LIVYWEMEDKNI	36. IVYWEMEDKNII
37. VYWEMEDKNIIQ	38. YWEMEDKNIIQF	39. WEMEDKNIIQFV	40. EMEDKNIIQFVH
41. MEDKNIIQFVHG	42. EDKNIIQFVHGE	43. DKNIIQFVHGEE	44. KNIIQFVHGEED
45. NIIQFVHGEEDL	46. IIQFVHGEEDLK	47. IQFVHGEEDLKV	48. QFVHGEEDLKVQ
49. FVHGEEDLKVQH	50. VHGEEDLKVQHS	51. HGEEDLKVQHSS	52. GEEDLKVQHSSY
53. EEDLKVQHSSYR	54. EDLKVQHSSYRQ	55. DLKVQHSSYRQR	56. LKVQHSSYRQRA
57. KVQHSSYRQRAR	58. VQHSSYRQRARL	59. QHSSYRQRARLL	60. HSSYRQRARLLK
61. SSYRQRARLLKD	62. SYRQRARLLKDQ	63. YRQRARLLKDQL	64. RQRARLLKDQLS
65. QRARLLKDQLSL	66. RARLLKDQLSLG	67. ARLLKDQLSLGN	68. RLLKDQLSLGNA
69. LLKDQLSLGNAA	70. LKDQLSLGNAAL	71. KDQLSLGNAALQ	72. DQLSLGNAALQI
73. QLSLGNAALQIT	74. LSLGNAALQITD	75. SLGNAALQITDV	76. LGNAALQITDVK
77. GNAALQITDVKL	78. NAALQITDVKLQ	79. AALQITDVKLQD	80. ALQITDVKLQDA
81. LQITDVKLQDAG	82. QITDVKLQDAGV	83. ITDVKLQDAGVY	84. TDVKLQDAGVYR
85. DVKLQDAGVYRC	86. VKLQDAGVYRCM	87. KLQDAGVYRCMI	88. LQDAGVYRCMIS
89. QDAGVYRCMISY	90. DAGVYRCMISYG	91. AGVYRCMISYGG	92. GVYRCMISYGGA
93. VYRCMISYGGAD	94. YRCMISYGGADY	95. RCMISYGGADYK	96. CMISYGGADYKR
97. MISYGGADYKRI	98. ISYGGADYKRIT	99. SYGGADYKRITV	100. YGGADYKRITVK
101. GGADYKRITVKV	102. GADYKRITVKVN	103. ADYKRITVKVNA	104. DYKRITVKVNAP
105. YKRITVKVNAPY	106. KRITVKVNAPYN	107. RITVKVNAPYNK	108. ITVKVNAPYNKI
109. TVKVNAPYNKIN	110. VKVNAPYNKINQ	111. KVNAPYNKINQR	112. VNAPYNKINQRI
113. NAPYNKINQRIL	114. APYNKINQRILV	115. PYNKINQRILVV	116. YNKINQRILVVD
117. NKINQRILVVDP	118. KINQRILVVDPV	119. INQRILVVDPVT	120. NQRILVVDPVTS
121. QRILVVDPVTSE	122. RILVVDPVTSEH	123. ILVVDPVTSEHE	124. LVVDPVTSEHEL
125. VVDPVTSEHELT	126. VDPVTSEHELTC	127. DPVTSEHELTCQ	128. PVTSEHELTCQA
129. VTSEHELTCQAE	130. TSEHELTCQAEG	131. SEHELTCQAEGY	132. EHELTCQAEGYP
133. HELTCQAEGYPK	134. ELTCQAEGYPKA	135. LTCQAEGYPKAE	136. TCQAEGYPKAEV
137. CQAEGYPKAEVI	138. QAEGYPKAEVIW	139. AEGYPKAEVIWT	140. EGYPKAEVIWTS
141. GYPKAEVIWTSS	142. YPKAEVIWTSSD	143. PKAEVIWTSSDH	144. KAEVIWTSSDHQ
145. AEVIWTSSDHQV	146. EVIWTSSDHQVL	147. VIWTSSDHQVLS	148. IWTSSDHQVLSG
149. WTSSDHQVLSGK	150. TSSDHQVLSGKT	151. SSDHQVLSGKTT	152. SDHQVLSGKTTT
153. DHQVLSGKTTTT	154. HQVLSGKTTTTN	155. QVLSGKTTTTNS	156. VLSGKTTTTNSK
157. LSGKTTTTNSKR	158. SGKTTTTNSKRE	159. GKTTTTNSKREE	160. KTTTTNSKREEK
161. TTTTNSKREEKL	162. TTTNSKREEKLF	163. TTNSKREEKLFN	164. TNSKREEKLFNV
165. NSKREEKLFNVT	166. SKREEKLFNVTS	167. KREEKLFNVTST	168. REEKLFNVTSTL
169. EEKLFNVTSTLR	170. EKLFNVTSTLRI	171. KLFNVTSTLRIN	172. LFNVTSTLRINT
173. FNVTSTLRINTT	174. NVTSTLRINTTT	175. VTSTLRINTTTN	176. TSTLRINTTTNE
177. STLRINTTTNEI	178. TLRINTTTNEIF	179. LRINTTTNEIFY	180. RINTTTNEIFYC
181. INTTTNEIFYCT	182. NTTTNEIFYCTF	183. TTTNEIFYCTFR	184. TTNEIFYCTFRR
185. TNEIFYCTFRRL	186. NEIFYCTFRRLD	187. EIFYCTFRRLDP	188. IFYCTFRRLDPE
189. FYCTFRRLDPEE	190. YCTFRRLDPEEN	191. CTFRRLDPEENH	192. TFRRLDPEENHT
193. FRRLDPEENHTA	194. RRLDPEENHTAE	195. RLDPEENHTAEL	196. LDPEENHTAELV
197. DPEENHTAELVI	198. PEENHTAELVIP	199. EENHTAELVIPE	200. ENHTAELVIPEL
201. NHTAELVIPELP	202. HTAELVIPELPL	203. TAELVIPELPLA	204. AELVIPELPLAH
205. ELVIPELPLAHP	206. LVIPELPLAHPP	207. VIPELPLAHPPN	208. IPELPLAHPPNE
209. PELPLAHPPNER	210. ELPLAHPPNERT	211. LPLAHPPNERTH	212. PLAHPPNERTHL
213. LAHPPNERTHLV	214. AHPPNERTHLVI	215. HPPNERTHLVIL	216. PPNERTHLVILG
217. PNERTHLVILGA	218. NERTHLVILGAI	219. ERTHLVILGAIL	220. RTHLVILGAILL
221. THLVILGAILLC	222. HLVILGAILLCL	223. LVILGAILLCLG	224. VILGAILLCLGV
225. ILGAILLCLGVA	226. LGAILLCLGVAL	227. GAILLCLGVALT	228. AILLCLGVALTF
229. ILLCLGVALTFI	230. LLCLGVALTFIF	231. LCLGVALTFIFR	232. CLGVALTFIFRL
233. LGVALTFIFRLR	234. GVALTFIFRLRK	235. VALTFIFRLRKG	236. ALTFIFRLRKGR
237. LTFIFRLRKGRM	238. TFIFRLRKGRMM	239. FIFRLRKGRMMD	240. IFRLRKGRMMDV
241. FRLRKGRMMDVK	242. RLRKGRMMDVKK	243. LRKGRMMDVKKC	244. RKGRMMDVKKCG
245. KGRMMDVKKCGI	246. GRMMDVKKCGIQ	247. RMMDVKKCGIQD	248. MMDVKKCGIQDT
249. MDVKKCGIQDTN	250. DVKKCGIQDTNS	251. VKKCGIQDTNSK	252. KKCGIQDTNSKK
253. KCGIQDTNSKKQ	254. CGIQDTNSKKQS	255. GIQDTNSKKQSD	256. IQDTNSKKQSDT
257. QDTNSKKQSDTH	258. DTNSKKQSDTHL	259. TNSKKQSDTHLE	260. NSKKQSDTHLEE
261. SKKQSDTHLEET

• Identify high antigenicity epitopes for antibody production & vaccine development
• Locate (auto)antigen epitopes of (auto)antibodies
• Define the best antibody clone against the protein-of-interest
• Perform epitope mutant analysis
• Map B cell epitopes

• Small serum sample volume required (2 µl)
• High density (simultaneously analyze hundreds of overlapping peptides)
• High sensitivity
• Large dynamic range
• Suitable for high-throughput assays
• High efficiency and accuracy
• Affordable, quick and simple to use

• RayBio^® human PD-L1 protein peptide array
• Blocking Buffer
• Wash Buffer I
• Wash Buffer II
• Biotin-conjugated secondary antibody
• Cy3 equivalent dye conjugated streptavidin
• Array accessories (including slide incubation chamber assembly, gasket, adhesive plastic strips, etc.)

• Distilled water
• Small plastic boxes or containers
• Orbital shaker or oscillating rocker
• Pipettors, pipette tips and other common lab consumables
• Aluminum foil
• Laser fluorescence scanner

View Compatible Laser Scanners

Don't have a compatible scanner? RayBiotech now offers FREE scanning service for all RayBio glass slide antibody arrays! Learn More

Upon receipt, all kit components should be stored at -20°C to -80°C until ready to use. Stored properly, the kit will retain complete activity for up to 6 months.

The full length of PD-L1 peptide array was detected using a biotin-conjugated polyclonal goat anti-human PD-L1 IgG antibody, following the kit’s user manual. The results indicate that several potential epitopes were revealed, exhibiting different signal densities at the central region and at the C-terminal (Figure 1, B & C).