Bovine Cytokine Array C1
RayBio® C-Series Bovine Cytokine Antibody Array 1 Kit. Detects 30 Bovine Cytokines. Suitable for all liquid sample types.
Product Description
Specifications
| Size | 2 Sample Kit, 4 Sample Kit, 8 Sample Kit |
|---|---|
| Species | Bovine (cow) |
| Number of Targets Detected | 30 |
| Quantitative/Semi-Quantitative | Semi-Quantitative |
| Compatible Sample Types | Cell Culture Supernatants, Plasma, Serum, Tissue Lysates, Cell Lysates |
| Solid Support | Membrane |
| Method Of Detection | Chemiluminescence |
| Design Principle | Sandwich-based |
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| Scroll over each target protein for more information | ||||
|---|---|---|---|---|
aFGF (FGF-1)
| Angiopoietin-1
| bFGF
| CD40 Ligand (TNFSF5)
| Decorin
|
GASP-1 (WFIKKNRP)
| IFN alpha
| IFN-beta
| IFN-gamma
| IGF-1
|
IL-1 alpha (IL-1 F1)
| IL-1 beta (IL-1 F2)
| IL-36 Ra (IL-1 F5)
| IL-10
| IL-13
|
IL-15
| IL-17A
| IL-18
| IL-2
| IL-21
|
IL-4
| IP-10 (CXCL10)
| LIF
| MCP-1 (CCL2)
| MIG (CXCL9)
|
MIP-1 beta (CCL4)
| NCAM-1 (CD56)
| RANTES (CCL5) | TNF alpha
| VEGF-A
|
Application Notes
Suggested Application
Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process
Kit Components
- Bovine Cytokine Antibody Array C1 Membranes
- Blocking Buffer
- Wash Buffer 1
- Wash Buffer 2
- Biotinylated Detection Antibody Cocktail
- Streptavidin-Conjugated HRP
- Detection Buffer C
- Detection Buffer D
- Lysis Buffer
- 8-Well Incubation Tray
- Plastic Sheets
- Array Templates
- Manual
Other Materials Required
- Pipettors, pipet tips and other common lab consumables
- Orbital shaker or oscillating rocker
- Tissue Paper, blotting paper or chromatography paper
- Adhesive tape or Saran Wrap
- Distilled or de-ionized water
- A chemiluminescent blot documentation system (such as UVP's ChemiDoc-It® or EpiChem II Benchtop Darkroom or GE's ImageQuant™ LAS 4000 or Amersham Imagers 600 and 680), X-ray Film and a suitable film processor, or another chemiluminescent detection system.
Protocol Outline
- Block membranes
- Incubate with Sample
- Incubate with Biotinylated Detection Antibody Cocktail
- Incubate with HRP-Conjugated Streptavidin
- Incubate with Detection Buffers
- Image with chemiluminescent imaging system
- Perform densitometry and analysis
Storage/Stability
For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months.
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