Product Features
- More cost-effective than traditional ELISA
- High specificity and system reproducibility
- Suitable for diverse sample types
- Low sample volume requirement: 50 µL or less
- Get results same day (6-hour processing time)
- Well-suited for high throughput assays
- Q Analyzer software provides one-step computation
Target Names
| Scroll over each target protein for more information |
|---|
aFGF (FGF-1)- Gene Symbol: FGF1
- Gene ID: 281160
| bFGF- Gene Symbol: FGF2
- Gene ID: 281161
- Accession #: P03969
| | IGF-1- Gene Symbol: IGF1
- Gene ID: 281239
- Accession #: P07455
| |
IL-4- Gene Symbol: IL4
- Gene ID: 280824
- Accession #: P30367
| IL-15- Gene Symbol: IL15
- Gene ID: 281248
- Accession #: Q28028
| MCP-1 (CCL2)- Gene Symbol: CCL2
- Gene ID: 281043
- Accession #: P28291
| NCAM-1 (CD56)- Gene Symbol: NCAM1
- Gene ID: 281941
- Accession #: P31836
| VEGF-A- Gene Symbol: VEGFA
- Gene ID: 281572
- Accession #: P15691
|
Application Notes
Suggested Application
- Multiplexed Protein Detection
- Biomarker Screening
- Identifying Key Factors
- Confirming Biological Process
- Biomarker Validation
- Validation of Antibody Array Results
- Quantitative Protein Detection
- Establishing Normal Range
Kit Components
- Bovine Cytokine Array Q2 Slide/s
- Blocking Buffer
- Wash Buffer 1
- Wash Buffer 2
- Lyophilized Standard Mix
- Biotinylated Detection Antibody Cocktail
- Streptavidin-Conjugated Fluor
- Slide Washer/Dryer
- Adhesive Plastic Strips
- Manual
Other Materials Required
- Distilled or deionized water
- Small plastic boxes or containers
- Pipettors, pipette tips and other common lab consumables
- Orbital shaker or oscillating rocker
- Aluminum foil
- Gene microarray scanner or similar laser fluorescence scanner
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Learn MoreProtocol Outline
- Dry the glass slide
- Prepare Standards
- Block array surface
- Incubate with Samples and Standards
- Incubate with Biotinylated Detection Antibody Cocktail
- Incubate with Streptavidin-Conjugated Fluor
- Disassemble the glass slide
- Scan with a gene microarray laser scanner
- Perform densitometry and analysis
Storage/Stability
For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array slide(s), standard mix, detection antibody cocktail, and Cy3-Conjugated Streptavidin at -20°C and all other reagents undiluted at 4°C for no more than 3 months.