Canine Cytokine Array C1

RayBio® C-Series Canine Cytokine Antibody Array 1 Kit. Detects 40 Canine Cytokines. Suitable for all liquid sample types.

$427.23
AAC-CYT-1-2
+

Antigen Information

Gene Symbols:
C1QTNF1 | CCL2 | CCL4 | CSF2 | CXCL1 | CXCL8 | DCN | EGFR | EPOR | FGF7   View more

Assay Format

Number of Targets Detected:
40
Species Detected:
  • Canine (dog)
Compatible Sample Types:
  • Cell Culture Supernatants
  • Cell Lysates
  • Plasma
  • Serum
  • Tissue Lysates
Design Principle:
  • Sandwich-based
Method of Detection:
Chemiluminescence
Quantitative/Semi-Quantitative:
Semi-Quantitative
Solid Support:
Membrane

Product Specifications

Size:
2, 4, or 8 Sample Kit

Product Features

  • Easy to use
  • No specialized equipment needed
  • Compatible with nearly any liquid sample
  • Proven technology (many publications)
  • Highly sensitive (pg/ml)
  • Sandwich ELISA specificity
  • Higher density than ELISA, Western blot or bead-based multiplex

Target Names

Scroll over each target protein for more information
Cystatin C
Decorin
EGFR
Erythropoietin
  • Gene Symbol: EPOR
  • Gene ID: 484943
  • Accession #: Q2KL21
FGF-7
(KGF)
  • Gene Symbol: FGF7 EGK_17479
  • Gene ID: 574345
  • Accession #: F7A1B0
Galectin-3
GASP-1
(WFIKKNRP)
GM-CSF
GRO alpha
(CXCL1)
HGF
HGFR
IFN-gamma
IGFBP-2
IL-1 alpha
(IL-1 F1)
IL-1 beta
(IL-1 F2)
IL-1 Ra
(IL-1 F3)
IL-10
IL-12 p40
IL-13
IL-17A
  • Gene Symbol: IL-17A IL17A
  • Gene ID: 481837
  • Accession #: C6L8D7
IL-2
IL-21
IL-6
IL-8
(CXCL8)
MCP-1
(CCL2)
MIP-1 beta
(CCL4)
  • Gene Symbol: CCL4 SCYA4
  • Gene ID: 448786
  • Accession #: Q68AZ0
NCAM-1
(CD56)
PDGF-BB
RAGE
RANTES
(CCL5)
Resistin
SCF
TGF alpha
TNF alpha
TNF RI
(TNFRSF1A)
  • Gene Symbol: TNFRSF1A
  • Gene ID: 403634;
  • Accession #: F1PNY7
Trappin-2
VEGF-A

Application Notes

Suggested Application
Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process
Kit Components
  • Canine Cytokine Antibody Array C1 Membranes
  • Blocking Buffer
  • Wash Buffer 1
  • Wash Buffer 2
  • Biotinylated Detection Antibody Cocktail
  • Streptavidin-Conjugated HRP
  • Detection Buffer C
  • Detection Buffer D
  • Lysis Buffer
  • 8-Well Incubation Tray
  • Plastic Sheets
  • Array Templates
  • Manual
Other Materials Required
  • Pipettors, pipet tips and other common lab consumables
  • Orbital shaker or oscillating rocker
  • Tissue Paper, blotting paper or chromatography paper
  • Adhesive tape or Saran Wrap
  • Distilled or de-ionized water
  • A chemiluminescent blot documentation system (such as UVP's ChemiDoc-It® or EpiChem II Benchtop Darkroom or GE's ImageQuant LAS 4000 or Amersham Imagers 600 and 680), X-ray Film and a suitable film processor, or another chemiluminescent detection system.
Protocol Outline
  1. Block membranes
  2. Incubate with Sample
  3. Incubate with Biotinylated Detection Antibody Cocktail
  4. Incubate with HRP-Conjugated Streptavidin
  5. Incubate with Detection Buffers
  6. Image with chemiluminescent imaging system
  7. Perform densitometry and analysis

Storage/Stability

For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months.

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Analysis Tool Bundle Discount

Specify Size
1 x $427.23
+
1 x $116.15 $34.84

70% off the data analysis tool when you purchase the array and tool together!


Note: If you are not placing this order online, make sure to mention this bundle discount to our orders department (orders@raybiotech.com).
Total list price $543.38
Price for all $462.07

Usage

This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.

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