Human Cytokine Array C4000

RayBio® C-Series Human Cytokine Antibody Array 4000 Kit. A combination of Human Cytokine Antibody Array C6, C7, C8, C9, & C10. Detects 274 Human Cytokines. Suitable for all liquid sample types.

AAH-CYT-4000
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Antigen Information

Gene Symbols:
ACE2 | ADAM17 | ADIPOQ | AFP | AGER | AGRP | ALCAM | ANG | ANGPT1 | ANGPT2   View more

Assay Format

Number of Targets Detected:
274
Species Detected:
  • Human
Compatible Sample Types:
  • Cell Culture Supernatants
  • Cell Lysates
  • Plasma
  • Serum
  • Tissue Lysates
Design Principle:
  • Sandwich-based
Method of Detection:
Chemiluminescence
Quantitative/Semi-Quantitative:
Semi-Quantitative
Solid Support:
Membrane

Product Specifications

Size:
2 or 4 Sample Kit

Product Features

  • Easy to use
  • No specialized equipment needed
  • Compatible with nearly any liquid sample
  • Proven technology (many publications)
  • Highly sensitive (pg/ml)
  • Sandwich ELISA specificity
  • Higher density than ELISA, Western blot or bead-based multiplex

Target Names

Scroll over each target protein for more information
4-1BB
(TNFRSF9/CD137)
ACE-2
  • Gene Symbol: ACE2
  • Gene ID: 59272
  • Accession #: Q9BYF1
Adiponectin
(ACRP30)
Activin A
Adipsin
(Complement Factor D)
AgRP
ALCAM
(CD166)
Alpha-fetoprotein
Amphiregulin
Angiogenin
Angiopoietin-1
Angiopoietin-2
Angiostatin
  • Gene Symbol: PLG
  • Gene ID: 5340
  • Accession #: P00747
ANGPTL4
Axl
CD80
(B7-1)
Beta-2 Microglobulin
BCAM
BCMA
(TNFRSF17)
BDNF
beta IG-H3
bFGF
BLC
(CXCL13)
BMP-4
BMP-5
BMP-6
BMP-7
beta-NGF
Betacellulin
(BTC)
CA125
CA15-3
CA19-9
CA9
Cardiotrophin-1
(CT-1)
Cathepsin S
HCC-1
(CCL14)
6Ckine
(CCL21)
CCL28
(MEC)
CD14
CD23
CD30
(TNFRSF8)
CD40
(TNFRSF5)
CD40 Ligand
(TNFSF5)
CEA
CEACAM-1
CK beta 8-1
(CCL23)
CNTF
Cripto-1
  • Gene Symbol: TDGF1
  • Gene ID: 6997
  • Accession #: P13385
CRP
(C-Reactive Protein)
CTACK
(CCL27)
CXCL16
DAN
Decorin
DKK-1
Dkk-3
Dkk-4
CD26
(DPPIV)
DR6
(TNFRSF21)
Dtk
E-Cadherin
EDA-A2
EGF
EGFR
EG-VEGF
(PK1)
ENA-78
(CXCL5)
Endoglin
(CD105)
Eotaxin-1
(CCL11)
Eotaxin-2
(MPIF-2/CCL24)
Eotaxin-3
(CCL26)
TROP1
(EpCAM)
ErbB2
ErbB3
Erythropoietin R
E-Selectin
Fas
(TNFRSF6/Apo-1)
Fas Ligand
(TNFSF6)
Fc gamma RIIB/C
(CD32B/C)
Ferritin
FGF-4
FGF-6
FGF-7
(KGF)
FGF-9
Flt-3 Ligand
FLRG
Follistatin
Fractalkine
(CX3CL1)
FSH
Furin
Galectin-7
GCP-2
(CXCL6)
GCSF
GDF-15
GDNF
GITR
(TNFRSF18)
GITR Ligand
(TNFSF18)
GM-CSF
GRO alpha/beta/gamma
GRO alpha
(CXCL1)
Growth Hormone
HB-EGF
HCC-4
(CCL16)
hCG intact
HGF
HVEM
(TNFRSF14)
I-309
(TCA-3/CCL1)
ICAM-1
(CD54)
ICAM-2
(CD102)
ICAM-3
(CD50)
IFN-gamma
IGFBP-1
IGFBP-2
IGFBP-3
IGFBP-4
IGFBP-6
IGF-1
IGF-1 R
IGF-2
  • Gene Symbol: Igf2
  • Gene ID: 3481
  • Accession #: P01344
IL-1 R2
IL-1 R4
(ST2)
IL-1 R1
IL-10
IL-10 R alpha
IL-10 R beta
IL-11
IL-12 p40
IL-12 p70
IL-13
IL-13 R alpha 2
IL-13 R1
IL-15
IL-16
IL-17A
  • Gene Symbol: IL17A
  • Gene ID: 3605
  • Accession #: Q16552
IL-17B
IL-17C
  • Gene Symbol: IL17C
  • Gene ID: 27189
  • Accession #: Q9P0M4
IL-17F
IL-17 RA
IL-18 BP alpha
IL-18 R beta
(AcPL)
IL-1 alpha
(IL-1 F1)
IL-1 beta
(IL-1 F2)
IL-1 ra
(IL-1 F3)
IL-2
IL-2 R beta
(CD122)
IL-2 R gamma
(Common gamma Chain)
  • Gene Symbol: IL2RG
  • Gene ID: 3561
  • Accession #: P31785
IL-2 R alpha
IL-21 R
IL-22
IL-28A
(IFN-lambda 2)
IL-29
(IFN-lambda 1)
IL-3
IL-31
IL-4
IL-5
IL-5 R alpha
IL-6
IL-6 R
IL-7
IL-8
(CXCL8)
IL-9
Insulin
IP-10
(CXCL10)
I-TAC
(CXCL11)
LAP/TGF beta 1
  • Gene Symbol: TGFB1
  • Gene ID: 7040
  • Accession #: P01137
Leptin
Leptin R
  • Gene Symbol: LEPR
  • Gene ID: 3953
  • Accession #: P48357
LIF
Light
(TNFSF14)
LIMPII
L-Selectin
(CD62L)
Luteinizing hormone
Lymphotactin
(XCL1)
LYVE-1
Marapsin
MCP-1
(CCL2)
MCP-2
(CCL8)
MCP-3
(MARC/CCL7)
MCP-4
(CCL13)
M-CSF
M-CSF R
MDC
(CCL22)
MICA
MICB
MIF
MIG
(CXCL9)
MIP-1 alpha
(CCL3)
MIP-1 beta
(CCL4)
MIP-1 delta
(CCL15)
MIP-3 alpha
(CCL20)
MIP-3 beta
(CCL19)
MMP-1
MMP-10
MMP-13
MMP-2
MMP-3
MMP-7
MMP-8
MMP-9
MPIF-1
(CCL23)
MSP alpha/beta
NAP-2
(PPBP/CXCL7)
NCAM-1
(CD56)
NGFR
(TNFRSF16)
Nidogen-1
NrCAM
NRG1-beta 1
(HRG1-beta 1)
NT-3
NT-4
Oncostatin M
Osteopontin
(SPP1)
Osteoprotegerin
(TNFRSF11B)
PAI-1
PARC
(CCL18)
PDGF-AA
PDGF R alpha
PDGF R beta
PDGF-AB
PDGF-BB
PECAM-1
(CD31)
PLGF
Platelet factor 4
(CXCL4)
Procalcitonin
Prolactin
  • Gene Symbol: PRL
  • Gene ID: 5617
  • Accession #: P01236
PSA-free
PSA-total
RAGE
RANK
(TNFRSF11A)
RANTES
(CCL5)
Resistin
S100 B
  • Gene Symbol: S100B
  • Gene ID: 6285
  • Accession #: P04271
SAA
(Serum Amyloid A)
SCF
SCF R
(CD117/c-kit)
SDF-1 alpha
(CXCL12 alpha)
SDF-1 beta
(CXCL12 beta)
gp130
Sonic Hedgehog N-Terminal
(Shh-N)
Siglec-5
(CD170)
Siglec-9
TNF RII
(TNFRSF1B)
TNF RI
(TNFRSF1A)
TACE
TARC
(CCL17)
TECK
(CCL25)
TGF beta 2
TGF alpha
TGF beta 3
  • Gene Symbol: TGFB3
  • Gene ID: 7043
  • Accession #: P10600
TGF beta 1
Thrombopoietin
(TPO)
Thyroglobulin
Tie-1
Tie-2
TIM-1
(KIM-1)
TIMP-1
TIMP-2
TIMP-4
TNF alpha
TNF beta
(TNFSF1B)
TRAIL R2
(TNFRSF10B/DR5)
TRAIL R3
(TNFRSF10C)
TRAIL R4
(TNFRSF10D)
Trappin-2
TREM-1
TSH
TSLP
Ubiquitin+1
uPAR
VCAM-1
(CD106)
VE-Cadherin
(CDH5)
  • Gene Symbol: CDH5
  • Gene ID: 1003
  • Accession #: P33151
VEGF-A
VEGFR2
VEGFR3
VEGF-C
VEGF-D
XEDAR
  • Gene Symbol: EDA2R
  • Gene ID: 60401
  • Accession #: Q9HAV5

Species Detected

Human

Application Notes

Suggested Application
Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process
Kit Components
  • Human Cytokine Antibody Array C4000 Membranes
  • Blocking Buffer
  • Wash Buffer 1
  • Wash Buffer 2
  • Biotinylated Detection Antibody Cocktail
  • Streptavidin-Conjugated HRP
  • Detection Buffer C
  • Detection Buffer D
  • Lysis Buffer
  • 8-Well Incubation Tray
  • Plastic Sheets
  • Array Templates
  • Manual
Other Materials Required
  • Pipettors, pipet tips and other common lab consumables
  • Orbital shaker or oscillating rocker
  • Tissue Paper, blotting paper or chromatography paper
  • Adhesive tape or Saran Wrap
  • Distilled or de-ionized water
  • A chemiluminescent blot documentation system (such as UVP's ChemiDoc-It® or EpiChem II Benchtop Darkroom or GE's ImageQuant LAS 4000 or Amersham Imagers 600 and 680), X-ray Film and a suitable film processor, or another chemiluminescent detection system.
Protocol Outline
  1. Block membranes
  2. Incubate with Sample
  3. Incubate with Biotinylated Detection Antibody Cocktail
  4. Incubate with HRP-Conjugated Streptavidin
  5. Incubate with Detection Buffers
  6. Image with chemiluminescent imaging system
  7. Perform densitometry and analysis

Storage/Stability

For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months.
I am super happy with the product and it is very detailed and easy to follow

University of Arizona
Easy to follow the proocol and it worked great

University of Arizona
This array was very easy to use. The protocol was easy to follow. I was able to detect signal using conditioned media from fibroblasts.
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Analysis Tool Bundle Discount

70% off the data analysis tool when you purchase the array and tool together!


Note: If you are not placing this order online, make sure to mention this bundle discount to our orders department (orders@raybiotech.com).

Usage

This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.

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