Human MMP Array C1

RayBio® C-Series Human Matrix Metalloproteinase Antibody Array 1 Kit. Detects 10 Human MMPs and TIMPs. Suitable for all liquid sample types.

$348.45
AAH-MMP-1-2
+

Antigen Information

Gene Symbols:
  • MMP1
  • MMP10
  • MMP13
  • MMP2
  • MMP3
  • MMP8
  • MMP9
  • TIMP1
  • TIMP2
  • TIMP4

Assay Format

Pathway:
  • Akt Signaling
  • HIF-1 alpha Signaling
Number of Targets Detected:
10
Species Detected:
  • Human
Compatible Sample Types:
  • Cell Culture Supernatants
  • Cell Lysates
  • Plasma
  • Serum
  • Tissue Lysates
Design Principle:
  • Sandwich-based
Method of Detection:
Chemiluminescence
Quantitative/Semi-Quantitative:
Semi-Quantitative
Solid Support:
Membrane

Product Specifications

Size:
4 or 8 Sample Kit

Product Features

  • Easy to use
  • No specialized equipment needed
  • Compatible with nearly any liquid sample
  • Proven technology (many publications)
  • Highly sensitive (pg/ml)
  • Sandwich ELISA specificity
  • Higher density than ELISA, Western blot or bead-based multiplex

Target Names

Scroll over each target protein for more information
MMP-1
MMP-2
MMP-3
MMP-8
MMP-9
MMP-10
MMP-13
TIMP-1
TIMP-2
TIMP-4

Application Notes

Suggested Application
Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process
Kit Components
  • Human Matrix Metalloproteinase Antibody Antibody Array C1 Membranes
  • Blocking Buffer
  • Wash Buffer 1
  • Wash Buffer 2
  • Biotinylated Detection Antibody Cocktail
  • Streptavidin-Conjugated HRP
  • Detection Buffer C
  • Detection Buffer D
  • Lysis Buffer
  • 8-Well Incubation Tray
  • Plastic Sheets
  • Array Templates
  • Manual
Other Materials Required
  • Pipettors, pipet tips and other common lab consumables
  • Orbital shaker or oscillating rocker
  • Tissue Paper, blotting paper or chromatography paper
  • Adhesive tape or Saran Wrap
  • Distilled or de-ionized water
  • A chemiluminescent blot documentation system (such as UVP's ChemiDoc-It® or EpiChem II Benchtop Darkroom or GE's ImageQuant LAS 4000 or Amersham Imagers 600 and 680), X-ray Film and a suitable film processor, or another chemiluminescent detection system.
Protocol Outline
  1. Block membranes
  2. Incubate with Sample
  3. Incubate with Biotinylated Detection Antibody Cocktail
  4. Incubate with HRP-Conjugated Streptavidin
  5. Incubate with Detection Buffers
  6. Image with chemiluminescent imaging system
  7. Perform densitometry and analysis

Storage/Stability

For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months.
  1. Chien YC., Liu LC., Ye HY., et al. EZH2 promotes migration and invasion of triple-negative breast cancer cells via regulating TIMP2-MMP-2/-9 pathway. Am J Cancer Res. 2018; 8(3): 422–434.PMCID: PMC5883093
    Species: Human
    Sample type: Conditioned Media
  2. Perrigue P., Silva M., Warden C., et al. The histone demethylase jumonji coordinates cellular senescence including secretion of neural stem cell-attracting cytokines. Mol Cancer Res. 2015 Apr;13(4):636-50. doi: 10.1158/1541-7786.MCR-13-0268
    Species: Human
    Sample type: Conditioned Media
  3. Salhi A., Farhadian J., Giles K., et al. RSK1 Activation Promotes Invasion in Nodular Melanoma. Am J Pathol. 2015 Mar;185(3):704-16. doi: 10.1016/j.ajpath.2014.11.021.
    Species: Human
    Sample type: Tissue Lysate
  4. Huang J., Yang B., Xiang T., et al. Diallyl disulfide inhibits growth and metastatic potential of human triple-negative breast cancer cells through inactivation of the ?-catenin signaling pathway. Mol Nutr Food Res. 2015 Jun;59(6):1063-75. doi: 10.1002/mnfr.201400668.
    Species: Human
    Sample type: Conditioned Media
  5. Carduner L., Leroy-Dudal J., Picot CR., Gallet O., Carreiras F., et al. Ascites-induced shift along epithelial-mesenchymal spectrum in ovarian cancer cells: enhancement of their invasive behavior partly dependant on ?v integrins. Clinical and Experimental Metastasis. Jun 2014 Epub Ahead of Print.
    Species: Human
    Sample type: Peritoneal Fluid
  6. Ichikawa T., Sugiura H., Koarai A., Minakata Y., Kikuchi T., Morishita Y., et al. TLR3 Activation Augments Matrix Metalloproteinase Production through Reactive Nitrogen Species Generation in Human Lung Fibroblasts. The Journal of Immunology April 23, 2014 1302919 [Epub Ahead of Print]
    Species: Human
    Sample type: Conditioned Media
  7. Krohn A., et al. Tumor Cell Heterogeneity in Small Cell Lung Cancer (SCLC): Phenotypical and Functional Differences Associated with Epithelial-Mesenchymal Transition (EMT) and DNA Methylation Changes. PLOS One Published: June 24, 2014 DOI: 10.1371/journal.pone.0100249
    Species: Human
    Sample type: Conditioned Media
  8. Orgaz J., et al. Diverse matrix metalloproteinase functionsregulate cancer amoeboid migration. Nature Communications 5, Article number:4255. doi:10.1038/ncomms5255
    Species: Human
    Sample type: Conditioned Media
  9. Patil P., Joshi M., Kuna Vijay., Xu B., Johannesson L., Olausson M., Sumitran-Holgersson S. CD271 identifies functional human hepatic stellate cells, which localize in peri-sinusoidal and portal areas in liver after partial hepatectomy. [Published same as previous]
    Species: Human
    Sample type: Conditioned Media
  10. Sakimoto T., et al. Simultaneous study of matrix metalloproteinases, proinflammatory cytokines, and soluble cytokine receptors in the tears of noninfectious corneal ulcer patients. Graefes Arch Clin Exp Ophthalmol Pubslished online July 2014. DOI 10.1007/s00417-014-2708-1
    Species: Human
    Sample type: Tears
In our experiment we are greatly satisfied with your kits. We were able to get clear and vivid pictures of MMP's and compare dots for different samples.

Stony Brook University (SUNY/Stony Brook Department of Ob/Gyn)
We have recently used the C-series array for the human MMPs on denatured protein isolated from skin extracts and it works great.
I like to emphasize that even though the kit recommends the use of non-denatured proteins using their supplied buffer, this worked fine for us, which a plus point to use this array.
The customer service and the scientific support team is excellent.
We will definitely use their products again.
Arup
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Analysis Tool Bundle Discount

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Note: If you are not placing this order online, make sure to mention this bundle discount to our orders department (orders@raybiotech.com).

Total list price $464.60
Price for all $383.29

Usage

This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.

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