Mouse Cytokine Array C1000

RayBio® C-Series Mouse Cytokine Antibody Array 1000. A combination of Mouse Cytokine Antibody Array C3 & C4. Detects 96 Mouse Cytokines. Suitable for all liquid sample types.

$611.05
AAM-CYT-1000-2
+

Antigen Information

Gene Symbols:
AXL | CCL1 | CCL11 | CCL12 | CCL17 | CCL19 | CCL2 | CCL20 | CCL22 | CCL24   View more

Assay Format

Number of Targets Detected:
96
Species Detected:
  • Mouse
Compatible Sample Types:
  • Cell Culture Supernatants
  • Cell Lysates
  • Plasma
  • Serum
  • Tissue Lysates
Design Principle:
  • Sandwich-based
Method of Detection:
Chemiluminescence
Quantitative/Semi-Quantitative:
Semi-Quantitative
Solid Support:
Membrane

Product Specifications

Size:
2, 4, or 8 Sample Kit

Product Features

  • Easy to use
  • No specialized equipment needed
  • Compatible with nearly any liquid sample
  • Proven technology (many publications)
  • Highly sensitive (pg/ml)
  • Sandwich ELISA specificity
  • Higher density than ELISA, Western blot or bead-based multiplex

Target Names

Scroll over each target protein for more information
Axl
bFGF
BLC
(CXCL13)
CD30 Ligand
(TNFSF8)
CD30
(TNFRSF8)
CD40
(TNFRSF5)
CRG-2
CTACK
(CCL27)
CXCL16
CD26
(DPPIV)
Dtk
Eotaxin-1
(CCL11)
Eotaxin-2
(MPIF-2/CCL24)
E-Selectin
Fas Ligand
(TNFSF6)
Fc gamma RIIB
(CD32b)
Flt-3 Ligand
Fractalkine
(CX3CL1)
GCSF
GITR
(TNFRSF18)
GM-CSF
HGFR
ICAM-1
(CD54)
IFN-gamma
IGFBP-2
IGFBP-3
IGFBP-5
IGFBP-6
IGF-1
IGF-2
IL-1 beta
(IL-1 F2)
IL-10
IL-12 p40/p70
IL-12 p70
  • Gene Symbol: IL12A
  • Accession #: P43431
IL-13
IL-15
IL-17A
  • Gene Symbol: IL17A
  • Accession #: Q60971
IL-17 RB
IL-1 alpha
(IL-1 F1)
IL-2
IL-3
IL-3 R beta
IL-4
IL-5
IL-6
IL-7
IL-9
I-TAC
(CXCL11)
KC
(CXCL1)
Leptin
Leptin R
LIX
L-Selectin
(CD62L)
Lungkine
(CXCL15)
Lymphotactin
(XCL1)
MCP-1
(CCL2)
MCP-5
M-CSF
MDC
(CCL22)
MIG
(CXCL9)
MIP-1 alpha
(CCL3)
MIP-1 gamma
MIP-2
MIP-3 beta
(CCL19)
MIP-3 alpha
(CCL20)
MMP-2
MMP-3
Osteopontin
(SPP1)
Osteoprotegerin
(TNFRSF11B)
Platelet Factor 4
(CXCL4)
Pro-MMP-9
P-Selectin
RANTES
(CCL5)
Resistin
SCF
SDF-1 alpha
(CXCL12 alpha)
Sonic Hedgehog N-Terminal
(Shh-N)
TNF RI
(TNFRSF1A)
TNF RII
(TNFRSF1B)
TARC
(CCL17)
I-309
(TCA-3/CCL1)
TECK
(CCL25)
TCK-1
(CXCL7)
TIMP-1
TIMP-2
TNF alpha
Thrombopoietin
(TPO)
TRANCE
(TNFSF11)
TROY
(TNFRSF19)
TSLP
VCAM-1
(CD106)
VEGF-A
VEGFR1
VEGFR2
VEGFR3
VEGF-D
  • Gene Symbol: FIGF
  • Accession #: A2AIH3

Species Detected

Mouse

Application Notes

Suggested Application
Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process
Kit Components
  • Mouse Cytokine Antibody Array C1000 Membranes
  • Blocking Buffer
  • Wash Buffer 1
  • Wash Buffer 2
  • Biotinylated Detection Antibody Cocktail
  • Streptavidin-Conjugated HRP
  • Detection Buffer C
  • Detection Buffer D
  • Lysis Buffer
  • 8-Well Incubation Tray
  • Plastic Sheets
  • Array Templates
  • Manual
Other Materials Required
  • Pipettors, pipet tips and other common lab consumables
  • Orbital shaker or oscillating rocker
  • Tissue Paper, blotting paper or chromatography paper
  • Adhesive tape or Saran Wrap
  • Distilled or de-ionized water
  • A chemiluminescent blot documentation system (such as UVP's ChemiDoc-It® or EpiChem II Benchtop Darkroom or GE's ImageQuant LAS 4000 or Amersham Imagers 600 and 680), X-ray Film and a suitable film processor, or another chemiluminescent detection system.
Protocol Outline
  1. Block membranes
  2. Incubate with Sample
  3. Incubate with Biotinylated Detection Antibody Cocktail
  4. Incubate with HRP-Conjugated Streptavidin
  5. Incubate with Detection Buffers
  6. Image with chemiluminescent imaging system
  7. Perform densitometry and analysis

Storage/Stability

For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months.
  1. Ivshina M., Alexandrov IM., Vertii A., et al. CPEB Regulation of TAK1 Synthesis Mediates Cytokine Production and the Inflammatory Immune Response. Mol Cell Biol. 2015 Feb 1;35(3):610-8. doi: 10.1128/MCB.00800-14
    Species: Mouse
    Sample type: Serum
  2. Arshad A, Deutsch E, Vozenin M-C (2015) Simultaneous Irradiation of Fibroblasts and Carcinoma Cells Repress the Secretion of Soluble Factors Able to Stimulate Carcinoma Cell Migration. PLoS ONE 10(1): e0115447. doi:10.1371/journal. pone.0115447
    Species: Mouse
    Sample type: Conditioned Media
  3. Grum-Schwensen B., Klingelhofer J., Beck M., et al. S100A4-neutralizing antibody suppresses spontaneous tumor progression, pre-metastatic niche formation and alters T-cell polarization balance. BMC Cancer. 2015; 15: 44. doi: 10.1186/s12885-015-1034-2
    Species: Mouse
    Sample type: Conditioned Media
  4. Yang Y., Gorzelanny C., Bauer AT., et al. Nuclear heparanase-1 activity suppresses melanoma progression via its DNA-binding affinity. Oncogene. 2015 Mar 9. doi: 10.1038/onc.2015.40.
    Species: Mouse
    Sample type: Tissue Lysate
  5. Taka E., Mazzio E., Goodman C., et al. Anti-inflammatory effects of thymoquinone in activated BV-2 microglial cells. J Neuroimmunol. 2015 Sep 15;286:5-12. doi: 10.1016/j.jneuroim.2015.06.011.
    Species: Mouse
    Sample type: Conditioned Media
  6. Jin H., Chen L., Zhang Z., et al. Deletion of angiotensin-converting enzyme 2 exacerbates renal inflammation and injury in apolipoprotein E-deficient mice through modulation of the nephrin and TNF-alpha-TNFRSF1A signaling. J Transl Med. 2015 Aug 6;13:255. doi: 10.1186/s12967-015-0616-8.
    Species: Mouse
    Sample type: Tissue Lysate
  7. Achyut B., Shankar A., Iskander A., et al. Bone marrow derived myeloid cells orchestrate antiangiogenic resistance in glioblastoma through coordinated molecular networks. Cancer Lett. 2015 Dec 28;369(2):416-26. doi: 10.1016/j.canlet.2015.09.004.
    Species: Mouse
    Sample type: Tissue Lysate
  8. Braig M., Pallman N., Preukschas M., Steinemann D., Hofmann W., et al. A ‘telomere-associated secretory phenotype’ cooperates with BCR-ABL to drive malignant proliferation of leukemic cells. 2014 Leukemia, Pubslihed April 1, Epub Ahead of Print, doi: 10.1038/leu.2014.95
    Species: Mouse
    Sample type: Conditioned Media
  9. Novitskiy et al.: Attenuated transforming growthfactor beta signaling promotes metastasis in a model of HER2mammary carcinogenesis. Breast Cancer Research 2014 16:425.\
    Species: Mouse
    Sample type: Conditioned Media
  10. Palmi C., et al. Cytoskeletal Regulatory Gene Expression and Migratory Properties of B Cell Progenitors are Affected by the ETV6-RUNX1 Rearrangement Mol Cancer Res molcanres.0056.2014; Published OnlineFirst July 24, 2014; doi: 10.1158/1541-7786.MCR-14-0056-T
    Species: Mouse
    Sample type: Conditioned Media
I have been using these arrays for years, and making great discoveries.

Florida A & M University
It is useful to find out the expression level of multiple cytokines from the same sample at same setting.

Augusta University
We ordered 100 customized membrane for 44 cytokines. It works really good.

Augusta University
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Analysis Tool Bundle Discount

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Note: If you are not placing this order online, make sure to mention this bundle discount to our orders department (orders@raybiotech.com).

Total list price $786.79
Price for all $663.77

Usage

This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.