Mouse Cytokine Array C1000

RayBio® C-Series Mouse Cytokine Antibody Array 1000. A combination of Mouse Cytokine Antibody Array C3 & C4. Detects 96 Mouse Cytokines. Suitable for all liquid sample types.

$611.05  
AAM-CYT-1000-2
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Assay Format

Number of Targets Detected:
96
Species Detected:
  • Mouse
Compatible Sample Types:
  • Cell Culture Supernatants
  • Cell Lysates
  • Plasma
  • Serum
  • Tissue Lysates
Design Principle:
  • Sandwich-based
Method of Detection:
Chemiluminescence
Quantitative/Semi-Quantitative:
  • Semi-Quantitative
Solid Support:
Membrane

Product Specifications

Size:
2, 4, or 8 Sample Kit

Protein Information

Gene Symbols:
AXL | CCL1 | CCL11 | CCL12 | CCL17 | CCL19 | CCL2 | CCL20 | CCL22 | CCL24   View more

Product Features

  • Easy to use
  • No specialized equipment needed
  • Compatible with nearly any liquid sample
  • Proven technology (many publications)
  • Highly sensitive (pg/ml)
  • Sandwich ELISA specificity
  • Higher density than ELISA, Western blot or bead-based multiplex

Target Names

Scroll over each target protein for more information
Axl
bFGF
BLC
(CXCL13)
CD30 Ligand
(TNFSF8)
CD30
(TNFRSF8)
CD40
(TNFRSF5)
CRG-2
CTACK
(CCL27)
CXCL16
CD26
(DPPIV)
Dtk
Eotaxin-1
(CCL11)
Eotaxin-2
(MPIF-2/CCL24)
E-Selectin
Fas Ligand
(TNFSF6)
Fc gamma RIIB
(CD32b)
Flt-3 Ligand
Fractalkine
(CX3CL1)
GCSF
GITR
(TNFRSF18)
GM-CSF
HGFR
ICAM-1
(CD54)
IFN-gamma
IGFBP-2
IGFBP-3
IGFBP-5
IGFBP-6
IGF-1
IGF-2
IL-1 beta
(IL-1 F2)
IL-10
IL-12 p40/p70
IL-12 p70
  • Gene Symbol: IL12A
  • Accession #: P43431
IL-13
IL-15
IL-17A
  • Gene Symbol: IL17A
  • Accession #: Q60971
IL-17 RB
IL-1 alpha
(IL-1 F1)
IL-2
IL-3
IL-3 R beta
IL-4
IL-5
IL-6
IL-7
IL-9
I-TAC
(CXCL11)
KC
(CXCL1)
Leptin
Leptin R
LIX
L-Selectin
(CD62L)
Lungkine
(CXCL15)
Lymphotactin
(XCL1)
MCP-1
(CCL2)
MCP-5
M-CSF
MDC
(CCL22)
MIG
(CXCL9)
MIP-1 alpha
(CCL3)
MIP-1 gamma
MIP-2
MIP-3 beta
(CCL19)
MIP-3 alpha
(CCL20)
MMP-2
MMP-3
Osteopontin
(SPP1)
Osteoprotegerin
(TNFRSF11B)
Platelet Factor 4
(CXCL4)
Pro-MMP-9
P-Selectin
RANTES
(CCL5)
Resistin
SCF
SDF-1 alpha
(CXCL12 alpha)
Sonic Hedgehog N-Terminal
(Shh-N)
TNF RI
(TNFRSF1A)
TNF RII
(TNFRSF1B)
TARC
(CCL17)
I-309
(TCA-3/CCL1)
TECK
(CCL25)
TCK-1
(CXCL7)
TIMP-1
TIMP-2
TNF alpha
Thrombopoietin
(TPO)
TRANCE
(TNFSF11)
TROY
(TNFRSF19)
TSLP
VCAM-1
(CD106)
VEGF-A
VEGFR1
VEGFR2
VEGFR3
VEGF-D
  • Gene Symbol: FIGF
  • Accession #: A2AIH3

Species Detected

Mouse

Application Notes

Suggested Application
Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process
Kit Components
  • Mouse Cytokine Antibody Array C1000 Membranes
  • Blocking Buffer
  • Wash Buffer 1
  • Wash Buffer 2
  • Biotinylated Detection Antibody Cocktail
  • Streptavidin-Conjugated HRP
  • Detection Buffer C
  • Detection Buffer D
  • Lysis Buffer
  • 8-Well Incubation Tray
  • Plastic Sheets
  • Array Templates
  • Manual
Other Materials Required
  • Pipettors, pipet tips and other common lab consumables
  • Orbital shaker or oscillating rocker
  • Tissue Paper, blotting paper or chromatography paper
  • Adhesive tape or Saran Wrap
  • Distilled or de-ionized water
  • A chemiluminescent blot documentation system (such as UVP's ChemiDoc-It® or EpiChem II Benchtop Darkroom or GE's ImageQuant LAS 4000 or Amersham Imagers 600 and 680), X-ray Film and a suitable film processor, or another chemiluminescent detection system.
Protocol Outline
  1. Block membranes
  2. Incubate with Sample
  3. Incubate with Biotinylated Detection Antibody Cocktail
  4. Incubate with HRP-Conjugated Streptavidin
  5. Incubate with Detection Buffers
  6. Image with chemiluminescent imaging system
  7. Perform densitometry and analysis

Storage/Stability

For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months.
  1. Li Z., Wang Z., Dinh P.U., et al. Cell-mimicking nanodecoys neutralize SARS-CoV-2 and mitigate lung injury in a non-human primate model of COVID-19. Nat Nanotechnol . 2021 Aug;16(8):942-951. doi: 10.1038/s41565-021-00923-2. Epub 2021 Jun 17.
    Species: Mouse
    Sample type: Serum (COVID-19 Lung Injury Model)
  2. Masaki T., Qu J., Cholewa-Waclaw J., Burr K., Raaum R., Rambukkan A., Reprogramming Adult Schwann Cells to Stem Cell-like Cells by Leprosy Bacilli Promotes Dissemination of Infection. Cell Volume 152, Issues 1-2, p51-67, 17 January 2013
    Species: Mouse
    Sample type: Conditioned Media (Conditioned Media)
  3. Ju, Zhenyu, et al. "Telomere dysfunction induces environmental alterations limiting hematopoietic stem cell function and engraftment." Nature medicine 13.6 (2007): 742.
    Species: Mouse
    Sample type: Plasma (Telomere dysfunction on HSC function)
  4. Murgai M., Ju W., Eason M., et al. KLF4-dependent perivascular cell plasticity mediates pre-metastatic niche formation and metastasis. Nat Med . 2017 Oct;23(10):1176-1190. doi: 10.1038/nm.4400. Epub 2017 Sep 18.
    Species: Mouse
    Sample type: Conditioned Media (B16-F0 and F10 cell lines in cancer metastasis model)
  5. Li, R., Huang, Y. & Lin, J. Distinct effects of general anesthetics on lung metastasis mediated by IL-6/JAK/STAT3 pathway in mouse models. Nat Commun 11, 642 (2020).
    Species: Mouse
    Sample type: Serum, Tissue Lysate (lung tissue lysate)
  6. Gómez-Aleza C, Nguyen B, Yoldi G, Ciscar M, Barranco A, Hernández-Jiménez E, Maetens M, Salgado R, Zafeiroglou M, Pellegrini P, Venet D, Garaud S, Trinidad EM, Benítez S, Vuylsteke P, Polastro L, Wildiers H, Simon P, Lindeman G, Larsimont D, Van den Eynden G, Velghe C, Rothé F, Willard-Gallo K, Michiels S, Muñoz P, Walzer T, Planelles L, Penninger J, Azim HA Jr, Loi S, Piccart M, Sotiriou C, González-Suárez E. Inhibition of RANK signaling in breast cancer induces an anti-tumor immune response orchestrated by CD8+ T cells. Nat Commun. 2020 Dec 10;11(1):6335. doi: 10.1038/s41467-020-20138-8. PMID: 33303745; PMCID: PMC7728758.
    Species: Mouse
    Sample type: Conditioned Media
  7. Stanford J. Efferocytosis produces a prometastatic landscape during postpartum mammary gland involution. JCI Sept 2014, epub ahead of print. doi:10.1172/JCI76375.
    Species: Mouse
    Sample type: Tissue Lysate (MPyV tumor lysate)
  8. Ijichi, Hideaki, et al. "Inhibiting Cxcr2 disrupts tumor-stromal interactions and improves survival in a mouse model of pancreatic ductal adenocarcinoma." The Journal of clinical investigation 121.10 (2011): 4106-4117.
    Species: Mouse
    Sample type: Conditioned Media (pancreatic tumor cells)
  9. Liu R., Chen S., Huang P., et al. Immunomodulation-Based Strategy for Improving Soft Tissue and Metal Implant Integration and Its Implications in the Development of Metal Soft Tissue Materials. Adv. Funct. Mater. 2020, 1910672
    Species: Mouse
    Sample type: Conditioned Media (M1 and M2 macrophage profiles with metal implants)
  10. Agrawal, N et al. Bacteriolytic therapy can generate a potent immune response against experimental tumors. Bacteriolytic therapy can generate a potent immune response against experimental tumors. Bacteriolytic therapy can generate a potent immune response against experimental tumors. Proc. Natl. Acad. Sci. U.S.A. Volume 101, Issue 42, Pages 15172-7, Oct 2004
    Species: Mouse
    Sample type: Conditioned Media (CD56, CD3+ cells)
Great product

The Ohio State University College of Medicine
I used this array for one of my researches and it was very helpful in detecting the impacted cytokines. I published the following paper:

Messeha SS, Zarmouh NO, Mendonca P, Kolta MG, Soliman KFA. The attenuating effects of plumbagin on pro-inflammatory cytokine expression in LPS-activated BV-2 microglial cells. Journal of neuroimmunology. 2017. Epub 2017/09/28. https://doi.org/10.1016/j.jneuroim.2017.09.007 PMID: 28950995.

Florida A & M University, Tallahassee, Florida
I have been using these arrays for years, and making great discoveries.

Florida A & M University
It is useful to find out the expression level of multiple cytokines from the same sample at same setting.

Augusta University
We ordered 100 customized membrane for 44 cytokines. It works really good.

Augusta University
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Usage

This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.

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