Name: Cathepsin S Activity Assay Kit (Fluorometric)
SKU: MA-CATHS-1
Availability: InStock

Product Description

Specifications

Size	1 Plate Kit, 2 Plate Kit, 5 Plate Kit
Estimated Lead Time	4-6 weeks
Compatible Sample Types	Tissue Lysates, Cell Lysates
Quantitative/Semi-Quantitative	Quantitative
Solid Support	96-well Microplate
Method Of Detection	Fluorometer
Shipping Type	Blue ice
Storage	-20°C

Kit Components

Component	Size / Description
Microplate	A 96-well plate
Assay Buffer	15 mL
Fluorescence Standard	1 vial
Cathepsin Substrate	1 vial
Cathepsin Positive Control	1 vial

Additional Materials Required

Microplate reader
Precision pipettes to deliver 2 µL to 1 mL volumes
Incubator at 37°C
Tubes to prepare sample dilutions
15 mL and 50 mL conical tubes
MilliQ water or another type of double distilled water (ddH₂O)

Assay Procedure Summary

Add 40 µL of samples, positive control, substrate background to the 96-well microtiter plate.
Add 10 µL assay buffer to each well.
Add 100 µL of standard to the 96-well microtiter plate.
Initiate the reaction by adding 50 µL diluted Cathepsin substrate solution to each well. Carefully shake the plate for a few seconds to mix well.
Measure fluorescence signal. For kinetic reading: Immediately start measuring fluorescence intensity continuously at 37°C and record data every 5 mins for 30 mins.

Storage/Stability

The entire kit should be stored below -20°C for up to 6 months from the date of shipment.

Kit Components

Component	Size / Description
Microplate	A 96-well plate
Assay Buffer	15 mL
Fluorescence Standard	1 vial
Cathepsin Substrate	1 vial
Cathepsin Positive Control	1 vial

Additional Materials Required

Microplate reader
Precision pipettes to deliver 2 µL to 1 mL volumes
Incubator at 37°C
Tubes to prepare sample dilutions
15 mL and 50 mL conical tubes
MilliQ water or another type of double distilled water (ddH₂O)

Assay Procedure Summary

Add 40 µL of samples, positive control, substrate background to the 96-well microtiter plate.
Add 10 µL assay buffer to each well.
Add 100 µL of standard to the 96-well microtiter plate.
Initiate the reaction by adding 50 µL diluted Cathepsin substrate solution to each well. Carefully shake the plate for a few seconds to mix well.
Measure fluorescence signal. For kinetic reading: Immediately start measuring fluorescence intensity continuously at 37°C and record data every 5 mins for 30 mins.

Storage/Stability

The entire kit should be stored below -20°C for up to 6 months from the date of shipment.

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Featured Products

Cathepsin S Activity Assay Kit (Fluorometric)

Product Description

Specifications

Kit Components

Additional Materials Required

Assay Procedure Summary

Storage/Stability

Documents

Have this product in hand?

Reviews

Ask A Question

Featured Products

Cathepsin S Activity Assay Kit (Fluorometric)

Product Description

Specifications

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Human Cathepsin S ELISA Kit

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Kit Components

Additional Materials Required

Assay Procedure Summary

Storage/Stability

Documents

Have this product in hand?

Reviews

Ask A Question

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