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Chymotrypsin, Alpha, TLCK Treated
Catalog #: 252-10110
Three times crystallized and treated with 1-chloro-3-tosylamido-7-amino-2-heptanone (TLCK) to inhibit trypsin activity (Shaw, et al., Biochemistry, 4, 2219, 1965). Dialyzed against 1 mM HCl to remove autolysis products and low molecular weight contam…
Product Description
Specifications
| Size | 25 mg, 100 mg, 1 gm |
| Estimated Lead Time | 1-2 weeks |
| Accession Number | P00766 |
| Expression System | Bovine Pancreas |
| Molecular Weight (kDa) | 25.6 kDa (Wilcox 1970) |
| Format | Lyophilized (powder) |
| Activity | ≥45 units per mg protein |
| Recommended Applications | Sequence analysis Peptide synthesis Peptide mapping Peptide fingerprinting, Sequence analysis Peptide synthesis Peptide mapping Peptide fingerprinting |
| Source | Bovine Pancreas |
| Shipping Type | Ambient temperature |
| Storage | 2-8°C |
Description
Other Information
Extinction Coefficient
51,840 cm-1 M-1 (Theoretical) E1%,280 Equals 20.19 (Chymotrypsinogen, Theoretical) E1%,280 Equals 20.57 (Chymotrypsin, Theoretical)
Isoelectric
8.52 (Chymotrypsinogen, Theoretical) 8.33 (Chymotrypsin, Theoretical)
CATH
Class: Mainly Beta Architecture: Beta Barrel Topology: Trypsin-like Serine Protease
CAS Registry Number
C.A.S.: 9004-07-3
Storage Conditions
Store at 2-8°C.