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Chymotrypsin, Alpha, TLCK Treated, Sequencing Grade

Catalog #: 252-10111

Three times crystallized and treated with 1-chloro-3-tosylamido-7-amino-2-heptanone (TLCK) to inhibit trypsin activity [Shaw, et al., Biochemistry, 4, 2219, (1965)]. Dialyzed to remove autolysis products and low molecular weight contaminants. Supplie…

Product Description

Specifications

Size4x25 µg, 4x100 µg
Estimated Lead Time1-2 weeks
Accession Number
P00766
Expression SystemBovine Pancreas
Molecular Weight (kDa)
25.6 kDa (Wilcox 1970)
Format
Lyophilized (powder)
Activity
≥45 units per milligram protein
Recommended ApplicationsSequence analysis Peptide synthesis Peptide mapping Peptide fingerprinting, Sequence analysis Peptide synthesis Peptide mapping Peptide fingerprinting
SourceBovine Pancreas
Shipping TypeAmbient temperature
Storage2-8°C

Description

Other Information

Protease sequencing grade
Yes
Extinction Coefficient
51,840 cm-1 M-1 (Theoretical) E1%,280 Equals 20.19 (Chymotrypsinogen, Theoretical) E1%,280 Equals 20.57 (Chymotrypsin, Theoretical)
Isoelectric
8.52 (Chymotrypsinogen, Theoretical) 8.33 (Chymotrypsin, Theoretical)
CATH
Class: Mainly Beta Architecture: Beta Barrel Topology: Trypsin-like Serine Protease
CAS Registry Number
C.A.S.: 9004-07-3
Storage Conditions
Store at 2-8°C