25% off all ELISA kits

Use code ELISA25-262 for 25% off all ELISAs through November.

25% off all ELISA kits

Use code ELISA25-262 for 25% off all ELISAs through November.

Citrullinated TNF alpha ELISA Kit

Measures citrullinated TNF alpha (TNFA) and total TNF alpha in human cell and tissue lysates, enabling precise assessment of TNF alpha citrullination by ELISA.

This assay can be developed for other species (mouse, rat, etc.) upon request. Contact techsupport@raybiotech.com.

Product Description

Specifications

Size	1 Plate Kit, 2 Plate Kit, 5 Plate Kit
Estimated Lead Time	4-8 weeks
Species	Human
Compatible Sample Types	Tissue Lysates, Cell Lysates
Accession Number	P01375
Gene Id	7124
Gene Symbols	TNF, TNFA, TNFSF2
Protein Name / Synonyms	Tumor necrosis factor; Cachectin; TNF-alpha; Tumor necrosis factor ligand superfamily member 2 (TNF-a)
Solid Support	96-well Microplate
Design Principle	Sandwich-based
Method Of Detection	Colorimetric
Research Area	Post-Translational Modifications, Epigenetics, Citrullination
Modification	Citrullination
Shipping Type	Blue ice
Storage	-20°C

Introduction

The RayBio^® Citrullinated-TNF alpha and Total-TNF alpha ELISA Kit is an in vitro enzyme-linked immunosorbent assay for the measurement of human citrullinated ATP-citrate synthase and total ATP-citrate synthase. An anti-pan TNF alpha antibody has been coated onto a 96-well plate. Samples are pipetted into the wells and TNF alpha present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-citrullination antibody is used to detect citrullinated TNF alpha or biotinylated anti-TNF alpha antibody is used to detect total TNF alpha . After washing away unbound antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of citrullinated TNF alpha or total TNF alpha bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

Product Features

Quantify quickly and easily
Screen numerous different lysates without performing a Western Blot analysis
Minimal hands-on time, convenient, and non-radioactive material

Kit Components

Component	Size / Qty
Microplate Coated With Anti-Pan-TNF alpha Antibody	1 plate
Anti-Citrullination Detection Antibody	1 vial
Anti-TNF alpha Detection Antibody	1 vial
HRP-Conjugated Streptavidin	200 µL
Wash Buffer	25 mL
Assay Diluent	15 mL
Lysis Buffer	5 mL
TMB One-Step Substrate Reagent	12 mL
Stop Solution	8 mL

Additional Materials Required

Microplate reader capable of measuring absorbance at 450 nm
Protease and Phosphatase inhibitors
Precision pipettes to deliver 2 µL to 1 mL volumes
Adjustable 1-25 mL pipettes for reagent preparation
100 mL and 1 liter graduated cylinders
Log-log graph paper or computer and software for ELISA data analysis
Absorbent paper
Tubes to prepare sample dilutions
Distilled or deionized water
Shaker

Assay Procedure Summary

Prepare all reagents and samples as instructed.
Add 100 µL sample to each well. Incubate 2.5 hours at room temperature or overnight at 4°C with gentle shaking.
Add 100 µL prepared detection antibody to each well. Incubate for 1 hour at room temperature with gentle shaking.
Add 100 µL prepared HRP-Conjugated Streptavidin solution. Incubate for 1 hour at room temperature with gentle shaking.
Add 100 µL TMB One-Step Substrate Reagent to each well. Incubate 30 minutes at room temperature.
Add 50 µL Stop Solution to each well. Read at 450 nm immediately.

Storage/Stability

The entire kit may be stored at -20°C for up to 6 months from the date of shipment. Avoid repeated freeze-thaw cycles.

Customization

This assay can be developed for other species (mouse, rat, etc.) upon request. Contact techsupport@raybiotech.com for more information.

Have this product in hand?

Get your lot-specific datasheet at Ray's Docs. The datasheet linked below is our current version and may not match your lot.

Excel (Sandwich-ELISA-Analysis-Tool.xlsm)

Introduction

Product Features

Quantify quickly and easily
Screen numerous different lysates without performing a Western Blot analysis
Minimal hands-on time, convenient, and non-radioactive material

Kit Components

Component	Size / Qty
Microplate Coated With Anti-Pan-TNF alpha Antibody	1 plate
Anti-Citrullination Detection Antibody	1 vial
Anti-TNF alpha Detection Antibody	1 vial
HRP-Conjugated Streptavidin	200 µL
Wash Buffer	25 mL
Assay Diluent	15 mL
Lysis Buffer	5 mL
TMB One-Step Substrate Reagent	12 mL
Stop Solution	8 mL

Additional Materials Required

Microplate reader capable of measuring absorbance at 450 nm
Protease and Phosphatase inhibitors
Precision pipettes to deliver 2 µL to 1 mL volumes
Adjustable 1-25 mL pipettes for reagent preparation
100 mL and 1 liter graduated cylinders
Log-log graph paper or computer and software for ELISA data analysis
Absorbent paper
Tubes to prepare sample dilutions
Distilled or deionized water
Shaker

Assay Procedure Summary

Prepare all reagents and samples as instructed.
Add 100 µL sample to each well. Incubate 2.5 hours at room temperature or overnight at 4°C with gentle shaking.
Add 100 µL prepared detection antibody to each well. Incubate for 1 hour at room temperature with gentle shaking.
Add 100 µL prepared HRP-Conjugated Streptavidin solution. Incubate for 1 hour at room temperature with gentle shaking.
Add 100 µL TMB One-Step Substrate Reagent to each well. Incubate 30 minutes at room temperature.
Add 50 µL Stop Solution to each well. Read at 450 nm immediately.

Storage/Stability

The entire kit may be stored at -20°C for up to 6 months from the date of shipment. Avoid repeated freeze-thaw cycles.

Customization

This assay can be developed for other species (mouse, rat, etc.) upon request. Contact techsupport@raybiotech.com for more information.

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25% off all ELISA kits

25% off all ELISA kits

Citrullinated TNF alpha ELISA Kit