Copper is an essential trace element. Copper containing enzymes play important roles in iron and catecholamine metabolism, free radical scavenging, and in the synthesis of hemoglobin, elastin, and collagen. Copper is mainly present in ceruloplasmin in the liver. Low levels of copper have been associated with mental retardation, depigmentation, anemia, hypotonia, and scorbutic changes in bone. Levels of copper are key diagnostic indicator of diseases such as Wilson's disease, microcytic hypochromic anemia, and bone disease due to reduced collagen synthesis.

Copper Assay Kit provides a simple, reproducible, and sensitive tool for measuring copper concentration in plasma, serum, cell lysates, saliva, urine, and other biological fluids without deproteinization of the sample. Dissociated copper from the complex by weakly acid buffer and reduced from Cu²⁺ to Cu⁺. The copper ion then reacts with 3, 5-DiBr-PAESA to form a purple complex which has a maximum absorption peak at 580 nm. The color intensity is proportional to the amount of copper present in the sample.

• Microplate reader capable of measuring absorbance at 580 nm
• Precision pipettes to deliver 2 µL to 1 mL volumes
• Tubes to prepare sample dilutions
• Multi-channel pipettes to deliver 20 µL to 200 µL volumes
• 15 ml, 50ml tubes

1. Add 100 µL of the diluted Copper Standards or samples to the 96-well microtiter plate.
2. Initiate the reaction by adding 150 µL Working Solution to all wells. Carefully shake the plate for a few seconds to mix.
3. Incubate the plate in the dark for 5 minutes at room temperature with mixing.
4. Measure the absorbance at580 nm using a plate reader.