25% off all ELISA kits

Use code ELISA25-262 for 25% off all ELISAs through November.

25% off all ELISA kits

Use code ELISA25-262 for 25% off all ELISAs through November.

COVID-19 Human IgA ELISA Kit

RayBio® COVID-19 N and S1 RBD protein Human Antibody Detection Kit (Indirect ELISA method) for the quantitative measurement of human IgA antibody against SARS-CoV-2 in serum. This Product is for Research Use Only. Not for use in in vitro diagnostics.

Product Description

Specifications

Size	1 Plate Kit, 2 Plate Kit, 5 Plate Kit
Estimated Lead Time	1-2 business days
Species	Human
Compatible Sample Types	Plasma, Serum, Dried Blood
Gene Symbols	IGA
Quantitative/Semi-Quantitative	Semi-Quantitative, Quantitative
Solid Support	96-well Microplate
Design Principle	Indirect
Method Of Detection	Colorimetric
Research Area	Infectious Disease, COVID-19
Shipping Type	Dry ice (additional shipping fee)
Storage	-80°C

Application Notes

Kit Components

SARS-CoV-2 N-protein coated 96-well microplate
Wash Buffer
Positive Control
Negative Control
HRP-anti positive control IgA Antibody
HRP-anti Human IgA Antibody
TMB One-Step Substrate
Stop Solution
Assay Diluent

Other Materials Required

Microplate reader capable of measuring absorbance at 450 nm
Precision pipettes to deliver 2 µl to 1 ml volumes
Adjustable 1-25 ml pipettes for reagent preparation
100 ml and 1 liter graduated cylinders
Absorbent paper
Distilled or deionized water

Protocol Outline

Prepare all reagents, samples and standards as instructed.
Add 100 μl positive control, negative control, or sample to each well. Incubate 1.5 hours at room temperature.
Add 100 μl prepared HRP-anti positive control IgA Antibody and/or 100 μl of prepared HRP-anti Human IgA Antibody to each well. Incubate 1 hour at room temperature.
Add 100 µl TMB One-Step Substrate Reagent to each well. Incubate 10 minutes at room temperature.
Add 50 µl Stop Solution to each well. Read at 450 nm immediately.

Storage/Stability

The entire kit may be stored at -20^°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. For extended storage, it is recommended to store at -80^°C.

Have this product in hand?

Get your lot-specific datasheet at Ray's Docs. The datasheet linked below is our current version and may not match your lot.

Datasheet (IE-CoVN-IgA.pdf)

Datasheet (IEQ-CoVS1RBD-IgA.pdf)

Datasheet (IEQ-CoVS1RBD-IgA-DBS.pdf)

Datasheet (IEQ-CoVSN-IgA.pdf)

Datasheet (IEQ-CoVSN-IgA-DBS.pdf)

Datasheet (IEQ-CoVSN-IgA-P.pdf)

SDS (IEQ-CoVSN-IgA.pdf)

SDS (IEQ-CoVSN-IgA-P.pdf)

SDS (IEQ-CoVSN-IgA-DBS.pdf)

SDS (IEQ-CoVS1RBD-IgA.pdf)

SDS (IEQ-CoVS1RBD-IgA-DBS.pdf)

SDS (IE-CoVN-IgA.pdf)

Application Notes

Kit Components

SARS-CoV-2 N-protein coated 96-well microplate
Wash Buffer
Positive Control
Negative Control
HRP-anti positive control IgA Antibody
HRP-anti Human IgA Antibody
TMB One-Step Substrate
Stop Solution
Assay Diluent

Other Materials Required

Microplate reader capable of measuring absorbance at 450 nm
Precision pipettes to deliver 2 µl to 1 ml volumes
Adjustable 1-25 ml pipettes for reagent preparation
100 ml and 1 liter graduated cylinders
Absorbent paper
Distilled or deionized water

Protocol Outline

Prepare all reagents, samples and standards as instructed.
Add 100 μl positive control, negative control, or sample to each well. Incubate 1.5 hours at room temperature.
Add 100 μl prepared HRP-anti positive control IgA Antibody and/or 100 μl of prepared HRP-anti Human IgA Antibody to each well. Incubate 1 hour at room temperature.
Add 100 µl TMB One-Step Substrate Reagent to each well. Incubate 10 minutes at room temperature.
Add 50 µl Stop Solution to each well. Read at 450 nm immediately.

Storage/Stability

The entire kit may be stored at -20^°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. For extended storage, it is recommended to store at -80^°C.

Good results on human breast milk
Rating
We used this kit for quantifying COVID-19 Human IgA in breast milk samples and were happy with the results. The background was too high for some samples but the problem can be solved by diluting them more and better separating the skim fraction.
Review by Liping Long, from Bond Life Sciences Center, on Oct 10, 2024

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25% off all ELISA kits

25% off all ELISA kits

COVID-19 Human IgA ELISA Kit