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25% off all ELISA kits

Use code ELISA25-262 for 25% off all ELISAs through November.

COVID-19 Human IgG ELISA Kit

RayBio® COVID-19 N and S1 RBD protein Human Antibody Detection Kit (Indirect ELISA method) for the quantitative measurement of human IgG antibody against SARS-CoV-2 in serum. This Product is for Research Use Only. Not for use in in vitro diagnostics.

Product Description

Specifications

Size	1 Plate Kit, 2 Plate Kit, 5 Plate Kit
Estimated Lead Time	1-2 business days
Species	Human
Compatible Sample Types	Plasma, Serum, Dried Blood, Saliva
Gene Symbols	IGG
Quantitative/Semi-Quantitative	Semi-Quantitative
Solid Support	96-well Microplate
Design Principle	Indirect
Method Of Detection	Colorimetric
Research Area	Infectious Disease, COVID-19
Shipping Type	Dry ice (additional shipping fee)
Storage	-80°C
Note	This Product is for Research Use Only, not for use in in vitro diagnostics, following guidance from the FDA for tests submitted to the FDA for Emergency Use Authorization and Notification on March 16th, 2020, and amended on May 4th, 2020. While this test has been submitted for FDA EUA Authorization, it currently has not been reviewed by the FDA and results from antibody testing should not be used as the sole basis to diagnose or exclude SARS-CoV-2 infection or to inform infection status. Please consult the manual for specific details and limitations of this test.

Typical Data

IEQ-CoVS1RBD-IgG1 was used to quantify anti-S1 receptor-binding domain IgG antibodies in plasma samples from cancer patients with COVID-19. Figure adapted from Remsik et al., Cancer Cell, 2021.

IEQ-CoVS1RBD-IgG-1 was used to quantify spike S1–specific IgG antibodies in matched maternal and fetal cord plasma from SARS-CoV-2–infected or vaccinated pregnancies. Figure adapted from Monroe et al., Journal of Infectious Diseases, 2024.

IEQ-CoVS1RBD-IgG-5 was used to quantify SARS-CoV-2 S1 RBD–specific IgG antibodies in serum from vaccinated animals, showing increased RBD-binding IgG titers after immunization. Figure adapted from Reguzova et al., NPJ Vaccines, 2024.

IEQ-CoVS1RBD-IgG-1 was used to quantify SARS-CoV-2 spike RBD levels in COVID-19 patient serum samples by ELISA. Figure adapted from Das et al., Microbiology and Immunology, 2024.

IEQ-CoVS1RBD-IgG-1 was used to quantify spike S1–specific IgG antibodies in matched maternal and fetal cord plasma collected at delivery from SARS-CoV-2–infected or vaccinated pregnancies. Figure adapted from Monroe et al., Journal of Infectious Diseases, 2024.

Kit Components

SARS-CoV-2 N-protein coated 96-well microplate
Wash Buffer
Positive Control
Biotinylated Anti-Human IgG
HRP-Streptavidin concentrate
TMB One-Step Substrate
Stop Solution
Assay Diluent
5X Sample Diluent
1X PBS (DBS version)
Tween-20 (DBS version)
Protease Inhibitor Cocktail (DBS version)

Additional Materials Required

Microplate reader capable of measuring absorbance at 450 nm
Precision pipettes to deliver 2 µL to 1 mL volumes
Adjustable 1-25 mL pipettes for reagent preparation
100 mL and 1 liter graduated cylinders
Tubes to prepare standard and sample dilutions
Absorbent paper
Distilled or deionized water

Assay Procedure Summary

Prepare all reagents, samples and standards as instructed.
Add 100 µL positive control or sample to each well. Incubate 1 hour at room temperature.
Add 100 μl prepared Biotinylated Anti-Human IgG Antibody into each well. Incubate 30 minutes at room temperature.
Add 100 μl prepared HRP-Streptavidin solution to each well. Incubate 30 minutes at room temperature.
Add 100 µL TMB One-Step Substrate Reagent to each well. Incubate 15 minutes at room temperature.
Add 50 µL Stop Solution to each well. Read at 450 nm immediately.

Storage/Stability

The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. For extended storage, it is recommended to store at -80°C.

Have this product in hand?

Get your lot-specific datasheet at Ray's Docs. The datasheet linked below is our current version and may not match your lot.

Datasheet (IEQ-CoVN-IgG.pdf)

Datasheet (IEQ-CoVN-IgG-DBS.pdf)

Datasheet (IEQ-CoVS1RBD-IgG.pdf)

Datasheet (IEQ-CoVS1RBD-IgG-DBS.pdf)

Datasheet (IEQ-CoVSN-IgG.pdf)

Datasheet (IEQ-CoVSN-IgG-DBS.pdf)

Datasheet (IEQ-CoVSN-IgG-P.pdf)

Datasheet (IEQ-CoVSN-IgG-S.pdf)

SDS (IEQ-CoVSN-IgG.pdf)

SDS (IEQ-CoVSN-IgG-P.pdf)

SDS (IEQ-CoVS1RBD-IgG.pdf)

SDS (IEQ-CoVN-IgG.pdf)

SDS (IEQ-CoVN-IgG-DBS.pdf)

SDS (IEQ-CoVSN-IgG-DBS.pdf)

SDS (IEQ-CoVSN-IgG-S.pdf)

SDS (IEQ-CoVS1RBD-IgG-DBS.pdf)

Typical Data

Kit Components

SARS-CoV-2 N-protein coated 96-well microplate
Wash Buffer
Positive Control
Biotinylated Anti-Human IgG
HRP-Streptavidin concentrate
TMB One-Step Substrate
Stop Solution
Assay Diluent
5X Sample Diluent
1X PBS (DBS version)
Tween-20 (DBS version)
Protease Inhibitor Cocktail (DBS version)

Additional Materials Required

Microplate reader capable of measuring absorbance at 450 nm
Precision pipettes to deliver 2 µL to 1 mL volumes
Adjustable 1-25 mL pipettes for reagent preparation
100 mL and 1 liter graduated cylinders
Tubes to prepare standard and sample dilutions
Absorbent paper
Distilled or deionized water

Assay Procedure Summary

Prepare all reagents, samples and standards as instructed.
Add 100 µL positive control or sample to each well. Incubate 1 hour at room temperature.
Add 100 μl prepared Biotinylated Anti-Human IgG Antibody into each well. Incubate 30 minutes at room temperature.
Add 100 μl prepared HRP-Streptavidin solution to each well. Incubate 30 minutes at room temperature.
Add 100 µL TMB One-Step Substrate Reagent to each well. Incubate 15 minutes at room temperature.
Add 50 µL Stop Solution to each well. Read at 450 nm immediately.

Storage/Stability

The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. For extended storage, it is recommended to store at -80°C.

Nice kit
Rating
Easy to use kit. Trained high school students how to process and test serum samples for CoV-2. Results were as expected for spike protein Ab'.
Review by Kelly, from Private, on Aug 7, 2023
Great product
Rating
We tested three plasma samples from vaccinated individuals as well as 6 donors or unkown vaccination status. The assay worked beautifully and we were able to confirm whether the plasma samples had measurable levels of anti-Spike protein IgG. Easy to use kit and great results.
Review by Daniel Rabe, from Massachusetts General Hospital, on Mar 9, 2021
Great product
Rating
Great Kit. I used it to screen mice sera for IgG against CoV-2 Spike protein. I was advised to use anti-Mouse-IgG-Biotin instead of anti-Human-IgG-Biotin. It worked very well. Definitely I recommends this kit.
Review by Ari, from Esperovax Inc, on Feb 9, 2021
Great product
Rating
Our laboratory was contracted to perform antibody testing for COVID19. We don't have the time or experience to develop an ELIZA method from scratch. By using and validating these kits. We were able to add a new service to our offerings within days. Using these kits, we were able to get repeatability and linearity between 3 different kits made on different days.
Review by Parimer Scientific, from Parimer Scientific - Private Lab, on Dec 22, 2020

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25% off all ELISA kits

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COVID-19 Human IgG ELISA Kit