Custom Antibody Service

RayBiotech offers a complete custom antibody service covering every step of monoclonal and polyclonal development. Our capabilities include peptide antigen design (including phospho peptides), synthesis and carrier protein conjugation, immunization, titer analysis, serum collection or hybridoma fusion, and antibody purification. Many customization options are available, such as antibody conjugation, lyophilization, custom vialing & aliquotting, and antibody validation by Western blot, ELISA, or immunoprecipitation.

Outline of Antibody Production Service

Service Items Polyclonal Ab (pAb) Monoclonal Ab (mAb)
Description Recognizing multiple antigenic sites of injected immunogen, immunization, high ELISA titer, purification Recognizing one antigenic binding site of the antigen, immunization, fusion, screening, subclone, characterization, purification
Size 150-900 kDa 150 kDa
Animal use Yes Yes
Period About 2 months 4-6 months
Specificity Low High
Affinity High Variable
Binding site Multiple Single
Amount Limited Reproducible
Service Items Time Description
Antibody Purification and Conjugation 2-3 days Conjugating polyclonal or monoclonal antibodies, with enzymes, fluorocrhomes, affinity ligand and solid surface conjugation

Part 1. Polyclonal Antibody

Service items Lead Time Description Deliverables
Anti-regular peptide polyclonal antibody (customer-provided peptide) 5 weeks
  1. Customer provides peptide: 1 mg non-conjugated; 2 mg KLH-conjugated (90% purity)
  2. Immunization of two rabbits
  3. Perform test bleeds, titer tests
  4. Purification of serum by protein A/G column
  5. Antigen affinity purification may be obtained upon request
10 mg purified antibody
Anti-regular peptide polyclonal antibody (we make peptide) 8 weeks
  1. Design, synthesis and conjugation of peptide (15-20-mer, 5-10 mg)
  2. Immunization of two rabbits
  3. Perform test bleeds, titer tests
  4. Purification of serum by protein A/G column
  5. Antigen affinity purification may be obtained upon request
10 mg purified antibody
Anti-phospho-peptide polylonal antibody 8-10 weeks
  1. Design, synthesis, and conjugation of phosphorylated peptide (15-20-mer, 5-10 mg)
  2. Immunization of two rabbits
  3. Perform test bleeds, titer tests
  4. Purification of serum by protein A/G column
  5. Antigen affinity purification may be obtained upon request
10 mg purified antibody
Anti-protein polyclonal antibody 5-7 weeks
  1. Customer provides 5 mg protein antigen
  2. Immunization of two rabbits
  3. Perform test bleeds, titer tests
  4. Purification of serum by protein A/G column
  5. Antigen affinity purification may be obtained upon request
10 mg purified antibody

Part 2. Monoclonal Antibody

Service Items Time Description
Anti-regular peptide antibody (customer provide antigen) 4-5 months
  1. Customer provides peptide (~4mg)
  2. Immunization of five mice, fusion, screening, subclone and initial characterization
  3. With up to three monoclones and their supernatant for confirming detection
  4. Generated antibody recognizes immunogen in ELISA assay
  5. Purify Antibody from 100 ml of hybridoma culture supernatant or 1 ml of ascites fluid
Anti-regular peptide antibody (RayBiotech provide antigen) 4-5 months
  1. Design, synthesize and conjugate peptide (15-20mer,5-10mg)
  2. Immunization of five mice, fusion, screening, subclone and initial characterization
  3. With up to three monoclones and their supernatant for confirming detection
  4. Generated antibody recognizes immunogen in ELISA assay
  5. Purify Antibody from 100 ml of hybridoma culture supernatant or 1 ml of ascites fluid
Anti-phospho-peptide antibody 5-6 months
  1. Design, synthesize, specific site phophorylation and conjugate peptide (11-15mer, 5 mg)
  2. Immunization of five mice, fusion, screening, subclone and initial characterization
  3. With up to three monoclones and their supernatant for confirming detection
  4. Generated antibody recognizes immunogen in ELISA assay
  5. Antibody purification by affinity column (1-5mg) may be obtained upon request
Anti-protein antibody 5 months
  1. 4mg of purified Protein from customer
  2. Immunize five mice, fusion, screening, subclone and initial characterization
  3. With up to three monoclones and their supernatant for confirming detection
  4. Generated antibody recognizes immunogen in ELISA assay
  5. Antibody purification by affinity column (1-5mg) may be obtained upon request