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Antibody Services

Leverage the same quality antibody production processes we put into our products for your custom research and bulk antibody production needs.

Our custom antibody services cover every step of the monoclonal and polyclonal development process and can include peptide antigen design (including phospho peptides), synthesis and carrier protein conjugation, immunization, titer analysis, serum collection or hybridoma fusion, and antibody purification.

We also offer a number of customizations, including antibody conjugation, lyophilization, custom vialing and aliquoting, and antibody validation by western blot, ELISA, or immunoprecipitation.

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Learn More About Our Different Antibody Services

Antibody Production
Antibody Conjugation Services
Recombinant Antibody Expression
Bulk Antibody Production
Epitope Mapping Services

Why You Should Choose Our Antibody Services

For Our Years of Experience

Founded in 2001 as the first company to commercialize the antibody array, we continue to use our decades of experience in multiplex, high-throughput protein analysis to deliver outstanding protein analysis and antibody-related services to the life science community.

What you can expect

Polyclonal Versus Monoclonal Antibody Production At-A-Glance

Polyclonal

Monoclonal

Production time

~2 Months

4-6 Months

Animal use

Yes

Yes

Antibody size

150 - 900 kDa

150 kDa

Avidity/Affinity

High (Avidity)

Variable (Affinity)

Binding sites recognized

Multiple

Single

Can be reproducibly produced

No

Yes

Antibody purification and conjugation

Our antibody purification and conjugation service typically takes 2-3 days and can include the conjugation of polyclonal or monoclonal antibodies to enzymes, fluorochromes, DNA, or affinity ligands as well as to solid supports.

Ready for RayBiotech to start working on your Antibody Service?

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FAQ

Frequently Asked Questions

Still have questions?

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We offer a diverse selection of over 18,000 monoclonal and polyclonal primary antibodies. RayBiotech's antibodies were developed to target multiple different proteins relevant to cell biology, neurobiology, immunology, virology, and many other fields. We’ve also developed secondary antibodies that target multiple host species immunoglobulin isotypes. These are available unlabeled or pre-conjugated to many different reporter systems, including alkaline phosphatase, biotin, FITC and other labels.

We can usually raise polyclonal antibodies against 150 to 900 kDa antigens in about 2 months. These wil be high affinity, low specificity antibodies, capable of binding multiple sites. Monoclonal antibodies can be raised against proteins no larger than 150 kDa in 4 to 6 months. These antibodies will be highly specific for only one binding site and their production can be scaled.

Still have questions?

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Related Services

Custom Protein Production

Comprehensive custom protein production service covers every step of production, from gene cloning through purification.

ELISA Services

Maximize operational efficiency while receiving data you can depend on when you trust your ELISA service needs to the experienced scientists at RayBiotech.

CRO Services

When you need a few extra hands to get an assay completed or support from experimental design to data analysis, our experienced team of scientists is ready to help.

Related Products

Proteins and Peptides

Whether you need a purified recombinant protein or peptide, overexpressed protein in cell extract or supernatant, a natively produced protein, or related reagents like protease inhibitors, you’ll find a quality product for confident discovery.

Antibodies

Choose from a wide range of pre-conjugated and unlabeled antibodies raised in a wide variety of species—all manufactured to the exacting standards you’ve come to expect from RayBiotech.

Arrays

Discover faster and develop deeper insights with simultaneous profiling of antibodies, proteins, peptides, and more—brought to you by the most experienced company in the business.

Flow Cytometry Reagents

Find quality reagents for flow cytometry-based insights including antibodies validated in flow cytometry studies, cell viability markers, intracellular staining kits, and more.

Polyclonals raised against a peptide

Option 1: Customer-provided peptide

Production time: 5 weeks

Amount: 10 mg purified antibody

Process:

You

  • Send us 1 mg non-conjugated or 2 mg KLH-conjugated (90% purity) peptide

RayBiotech

  • Immunizes two rabbits (other species available)
  • Performs test bleeds, titer tests
  • Purifies serum by protein A/G column
  • Performs antigen affinity purification if requested
Option 2: RayBiotech synthesizes the peptide

Production time: 8 weeks

Amount: 10 mg purified antibody

Process:

RayBiotech

  • Designs, synthesizes and conjugates the peptide antigen (15-20-mer, 5-10 mg)
  • Immunizes two rabbits (other species available)
  • Performs test bleeds, titer tests
  • Purifies serum by protein A/G column
  • Performs antigen affinity purification if requested

Polyclonals raised against a phospho-peptide

Production time: 8-10 weeks

Amount: 10 mg purified antibody

Process:

RayBiotech

  • Designs, synthesizes and conjugates the phosphorylated peptide antigen (15-20-mer, 5-10 mg)
  • Immunizes two rabbits (other species available)
  • Performs test bleeds, titer tests
  • Purifies serum by protein A/G column
  • Performs antigen affinity purification if requested

Polyclonals raised against a protein

Production time: 5-7 weeks

Amount: 10 mg purified antibody

Process:

You

  • Send us 5 mg of protein antigen

RayBiotech

  • Immunizes two rabbits (other species available)
  • Performs test bleeds, titer tests
  • Purifies serum by protein A/G column
  • Performs antigen affinity purification if requested

Polyclonals raised against a peptide

Option 1: Customer-provided peptide

Production time: 5 weeks

Amount: 10 mg purified antibody

Process:

You

  • Send us 1 mg non-conjugated or 2 mg KLH-conjugated (90% purity) peptide

RayBiotech

  • Immunizes two rabbits (other species available)
  • Performs test bleeds, titer tests
  • Purifies serum by protein A/G column
  • Performs antigen affinity purification if requested
Option 2: RayBiotech synthesizes the peptide

Production time: 8 weeks

Amount: 10 mg purified antibody

Process:

RayBiotech

  • Designs, synthesizes and conjugates the peptide antigen (15-20-mer, 5-10 mg)
  • Immunizes two rabbits (other species available)
  • Performs test bleeds, titer tests
  • Purifies serum by protein A/G column
  • Performs antigen affinity purification if requested

Polyclonals raised against a phospho-peptide

Production time: 8-10 weeks

Amount: 10 mg purified antibody

Process:

RayBiotech

  • Designs, synthesizes and conjugates the phosphorylated peptide antigen (15-20-mer, 5-10 mg)
  • Immunizes two rabbits (other species available)
  • Performs test bleeds, titer tests
  • Purifies serum by protein A/G column
  • Performs antigen affinity purification if requested

Polyclonals raised against a protein

Production time: 5-7 weeks

Amount: 10 mg purified antibody

Process:

You

  • Send us 5 mg of protein antigen

RayBiotech

  • Immunizes two rabbits (other species available)
  • Performs test bleeds, titer tests
  • Purifies serum by protein A/G column
  • Performs antigen affinity purification if requested

Monoclonals raised against a peptide

Option 1: Customer-provided peptide

Production time: 4-5 months

Process:

You

  • Send us 4 mg of peptide antigen

RayBiotech

  • Immunizes up to five mice and performs fusion, screening, subcloning, and initial characterization
  • Identifies up to three monoclones and their supernatant to confirm detection
  • Verifies immunogen recognition using an ELISA
  • Purifies monoclonal from 100 mL of hybridoma culture supernatant or 1 mL of ascites fluid
Option 2: RayBiotech synthesizes the peptide

Production time: 4-5 months

Process:

RayBiotech

  • Designs, synthesizes and conjugates the peptide antigen (15-20-mer, 5-10 mg)
  • Immunizes up to five mice and performs fusion, screening, subcloning, and initial characterization
  • Identifies up to three monoclones and their supernatant to confirm detection
  • Verifies immunogen recognition using an ELISA
  • Purifies monoclonal from 100 mL of hybridoma culture supernatant or 1 mL of ascites fluid

Monoclonals raised against a phospho-peptide

Production time: 5-6 months

Process:

RayBiotech

  • Designs, synthesizes and conjugates the peptide antigen (15-20-mer, 5-10 mg)
  • Immunizes up to five mice and performs fusion, screening, subcloning, and initial characterization
  • Identifies up to three monoclones and their supernatant to confirm detection
  • Verifies immunogen recognition using an ELISA
  • Purifies monoclonal from 100 mL of hybridoma culture supernatant or 1 mL of ascites fluid

Monoclonals raised against a protein

Production time: 5 months

Process:

You

  • Send us 4 mg of purified protein antigen

RayBiotech

  • Immunizes up to five mice and performs fusion, screening, subcloning, and initial characterization
  • Identifies up to three monoclones and their supernatant to confirm detection
  • Verifies immunogen recognition using an ELISA
  • Purifies monoclonal from 100 mL of hybridoma culture supernatant or 1 mL of ascites fluid

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