Product Feature
- Easy to use
- No specialized equipment needed
- Compatible with nearly any liquid sample
- Proven technology (many publications)
- Highly sensitive (pg/ml)
- Sandwich ELISA specificity
- Higher density than ELISA, Western blot or bead-based multiplex
Target Names Available
4-1BB, 6Ckine, ACE, ALK-1, Amphiregulin, Axl, bFGF, BLC, Cardiotrophin-1, CCL1, CCL11, CCL17, CCL19, CCL2, CCL20, CCL22, CCL24, CCL25, CCL27, CCL3, CCL5, CD105, CD106, CD152, CD26, CD27, CD27 Ligand, CD30, CD30 Ligand, CD32b, CD36, CD40, CD40 Ligand, CD54, CD62L, Chordin, CRG-2, CTACK, CTLA-4, CX3CL1, CXCL1, CXCL11, CXCL12, CXCL12 alpha, CXCL16, CXCL4, CXCL9, Decorin, DKK-1, DPPIV, Dtk, E-Cadherin, EGF, Endoglin, Eotaxin-1, Eotaxin-2, Epigen, Epiregulin, E-Selectin, Fas Ligand, Fc gamma RIIB, Flt-3 Ligand, Fractalkine, Galectin-1, Gas 1, Gas 6, G-CSF, GITR, GITR Ligand, GM-CSF, Granzyme B, HAI-1, HGF, HGFR, ICAM-1, IFN-gamma, IFN-lambda, IGF-1, IGF-2, IGFBP-2, IGFBP-3, IGFBP-5, IGFBP-6, IL-1 alpha, IL-1 beta, IL-1 F3, IL-1 R4, IL-1 Ra, IL-10, IL-11, IL-12 p40, IL-12 p40/p70, IL-12 p70, IL-13, IL-15, IL-17, IL-17B, IL-17B R, IL-17E, IL-17F, IL-1F1, IL-1F2, IL-2, IL-2 R alpha, IL-20, IL-21, IL-28, IL-3, IL-3 R beta, IL-4, IL-5, IL-6, IL-6 R, IL-7, IL-9, I-TAC, JAM-A, KC, L-17F, Leptin, Leptin R, LIX, L-Selectin, Lungkine, Lymphotactin, MAdCAM-1, MCP-1, MCP-5, M-CSF, MDC, MFG-E8, MIG, MIP-1 alpha, MIP-1 gamma, MIP-2, MIP-3 alpha, MIP-3 beta, MMP-2, MMP-3, MPIF-2, Neprilysin, Osteopontin, Osteoprotegerin, Pentraxin-3, Platelet Factor 4, Prolactin, Pro-MMP-9, P-Selectin, RAGE, RANTES, Resistin, SCF, SDF-1, SDF-1 alpha, Sonic Hedgehog N-Terminal, SPP1, SR-B3, ST2, sTNFRI, sTNFRII, TACI, TARC, TECK, Thrombopoietin, Thymus CK-1, TIMP-1, TIMP-2, TNF alpha, TNFRSF11B, TNFRSF12, TNFRSF13B, TNFRSF18, TNFRSF19, TNFRSF5, TNFRSF7, TNFRSF8, TNFSF11, TNFSF12, TNFSF18, TNFSF5, TNFSF6, TNFSF7, TNFSF8, TPO, TRANCE, TREM-1, TROY, TSG-14, TSLP, TWEAK, TWEAK R, VCAM-1, VEGF-A, VEGF-D, VEGFR1, VEGFR2, VEGFR3, XCL1
Target Species
Mouse
Application Notes
Suggested Application
Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process; Detecting Nephrotoxicity
Kit Components
- RayBio® Custom C-Series Mouse Cytokine Antibody Array Membranes
- Blocking Buffer
- Wash Buffer 1
- Wash Buffer 2
- Biotinylated Detection Antibody Cocktail
- Streptavidin-Conjugated HRP
- Detection Buffer C
- Detection Buffer D
- Lysis Buffer
- 8-Well Incubation Trays
- Plastic Sheets
- Array Templates
- Manual
Other Materials Required
- Pipettors, pipet tips and other common lab consumables
- Orbital shaker or oscillating rocker
- Tissue Paper, blotting paper or chromatography paper
- Adhesive tape or Saran Wrap
- Distilled or de-ionized water
- A chemiluminescent blot documentation system (such as UVP??s ChemiDoc-It® or EpiChem II Benchtop Darkroom or GE's ImageQuant™ LAS 4000 or Amersham Imagers 600 and 680), X-ray Film and a suitable film processor, or another chemiluminescent detection system.
Protocol Outline
- Block membranes
- Incubate with Sample
- Incubate with Biotinylated Detection Antibody Cocktail
- Incubate with HRP-Conjugated Streptavidin
- Incubate with Detection Buffers
- Image with chemiluminescent imaging system
- Perform densitometry and analysis
Storage/Stability
For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months.