RayBio® Human ANGPTL3 ELISA Kit for serum, plasma, and cell culture supernatant samples.
Lead time: Typically ships within 1-2 business days. No Friday shipments.
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Product Description
Specifications
| Size | 1 Plate Kit, 2 Plate Kit, 5 Plate Kit |
| Estimated Lead Time | 1-2 business days |
| Species | Human |
| Sensitivity | 8.2 pg/ml Need more sensitivity? Check out the new NexaTag® kit for this target. Still not enough? Then your answer is our Ultrasensitive Biomarker Testing Service powered by Simoa™ technology. |
| Detection Range | 8.2 pg/ml - 2000 pg/ml |
| Specificity | This ELISA kit shows no cross-reactivity with the following cytokines tested: human beta IG-H3, CA9, Cathepsin B, CD23, CD26 (DPPIV), CHI3L1, Coagulation Factor III, CTLA-4, Dkk-4, EDA-A2, Erythropoietin R, FGF-6, FGF-9, FOLH1 (PSMA), Gas 1, IGFBP-5, IL-38 (IL-1 F10), IL-36 Ra (IL-1 F5), IL-36 alpha (IL-1 F6), IL-37 (IL-1 F7), IL-36 beta (IL-1 F8), IL-36 gamma (IL-1 F9), IL-1 R5 (IL-18 R alpha), IL-10 R alpha, IL-17C, IL-18, IL-20, IL-34, IL-5 R alpha, Layilin, Leptin R, Marapsin, Mer, MMP-7, P-Cadherin, Prostasin, SIGIRR, TGF beta R3,TWEAK. |
| Compatible Sample Types | Cell Culture Supernatants, Plasma, Serum |
| Recommended Dilution (Serum/Plasma) | 200 fold |
| Accession Number | Q9Y5C1 |
| Gene Id | 27329 |
| Gene Symbols | ANGPTL3, ANGPT5, UNQ153/PRO179 |
| Protein Name / Synonyms | Angiopoietin-related protein 3 (Angiopoietin-5) (ANG-5) (Angiopoietin-like protein 3) |
| Quantitative/Semi-Quantitative | Quantitative |
| Solid Support | 96-well Microplate |
| Design Principle | Sandwich-based |
| Method Of Detection | Colorimetric |
| Research Area | Angiogenesis, Vascular Biology |
| Shipping Type | Blue ice |
| Storage | ≤-20°C |
Product Features
Additional Notes
- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
Standard Curves
Spiking & Recovery Results
Linearity Results
Application Notes
Kit Components
- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
Other Materials Required
- Distilled or deionized water
- Precision pipettes to deliver 2 µl to 1 µl volumes
- Adjustable 1-25 µl pipettes for reagent preparation
- 100 µl and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
Protocol Outline
- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 µl of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4°C.
- Add 100 µl of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 µl of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 µl of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 µl of Stop Solution to each well.
- Read at 450 nm immediately.
Storage/Stability
The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
