RayBio® Mouse C5a ELISA Kit for cell culture supernatants, plasma, and serum samples.
Lead time: Typically ships within 1-2 business days. No Friday shipments.
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Product Description
Specifications
| Size | 1 Plate Kit, 2 Plate Kit, 5 Plate Kit |
| Estimated Lead Time | 1-2 business days |
| Species | Mouse |
| Sensitivity | 1.5 pg/ml Need more sensitivity? Check out the new NexaTag® kit for this target. Still not enough? Then your answer is our Ultrasensitive Biomarker Testing Service powered by Simoa™ technology. |
| Detection Range | 1.5 pg/ml - 400 pg/ml |
| Specificity | This ELISA kit shows no cross-reactivity with the following cytokines tested: Mouse BLC, CD27, CD30 Ligand, Eotaxin, Eotaxin-2, Fas Ligand, G-CSF, GM-CSF, ICAM-1, IFN-gamma, IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-10, IL-12 p40, IL-13, IL-15, IL-17, IL-21, KC, Leptin, LIX, MCP-1, MCP-5, M-CSF, MIG, MIP-1 alpha, MIP-1 gamma, PF-4, RANTES, TARC, TCA-3, TNF alpha, TNFRI, VEGF. |
| Compatible Sample Types | Cell Culture Supernatants, Plasma, Serum |
| Recommended Dilution (Serum/Plasma) | 100 - 1,000 fold for serum and EDTA plasma; 10 fold for citrate/heparin plasma. |
| Accession Number | P06684 |
| Gene Id | 15139 |
| Gene Symbols | C5, HC |
| Protein Name / Synonyms | Complement C5 (Hemolytic complement) [Cleaved into: Complement C5 beta chain Complement C5 alpha chain C5a anaphylatoxin Complement C5 alpha' chain] |
| Quantitative/Semi-Quantitative | Quantitative |
| Solid Support | 96-well Microplate |
| Design Principle | Sandwich-based |
| Method Of Detection | Colorimetric |
| Research Area | Angiogenesis, Inflammation |
| Shipping Type | Blue ice |
| Storage | ≤-20°C |
Target Background
C5a and C5b are protein fragments released from the cleavage of complement component 5 by the C5-convertase protease. C5a is a highly inflammatory peptide that is involved in complement activation, attracting of innate immune cells and histamine release. Abnormal activation of inflammatory complement fragments such as C3a, C4a and C5a can lead to inflammatory disease. C5a binds to the C5a receptor to mediate a variety biological activity. Eculizumab is a monoclonal antibody that binds complement protein C5, inhibiting its cleavage into C5a and C5b. Eculizumab is used to reduce the risk of hemolysis and paroxysmal nocturnal hemoglobinuria.
Product Features
Additional Notes
- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
Standard Curves
Spiking & Recovery Results
Linearity Results
Application Notes
Kit Components
- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
Other Materials Required
- Distilled or deionized water
- Precision pipettes to deliver 2 µl to 1 µl volumes
- Adjustable 1-25 µl pipettes for reagent preparation
- 100 µl and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
Protocol Outline
- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 µl of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4°C.
- Add 100 µl of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 µl of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 µl of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 µl of Stop Solution to each well.
- Read at 450 nm immediately.
Storage/Stability
The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
