Product Feature
- More cost-effective than traditional ELISA
- High specificity and system reproducibility
- Suitable for diverse sample types
- Low sample volume requirement: 50 µL or less
- Get results same day (6-hour processing time)
- Well-suited for high throughput assays
- Q Analyzer software provides one-step computation
Target Names Available
beta-NGF, CINC-2, CINC-3, CNTF, CX3CL1, Fractalkine, GM-CSF, IL-1 alpha, IL-1F1, IL-4, LIX, VEGF-A, CCL2, CD54, ICAM-1, IFN-gamma, IL-1 beta, IL-10, IL-6, L-Selectin, Leptin, MCP-1, TIMP-1, TNF alpha, b-NGF, B7-2, CCL2, CD54, CD62L, CINC-1, CINC-2, CINC-3, CNTF, CX3CL1, Fractalkine, GM-CSF, ICAM-1, IFN-gamma, IL-1 alpha, IL-1 beta, IL-10, IL-13, IL-1F1, IL-1F2, IL-2, IL-4, IL-6, L-Selectin, Leptin, LIX, MCP-1, PDGF-AA, Prolactin R, RAGE, TCK-1, TIMP-1, TNF alpha, VEGF-A, CCL2, IFN-gamma, IL-1 alpha, IL-1 beta, IL-10, IL-13, IL-1F1, IL-1F2, IL-2, IL-4, IL-6, MCP-1, TNF alpha
Target Species
Rat
Application Notes
Suggested Application
Multiplexed Protein Detection; Biomarker Screening; Identifying Key Factors; Confirming Biological Process; Biomarker Validation; Validation of Antibody Array Results; Quantitative Protein Detection; Establishing Normal Range; Isotyping
Kit Components
- Custom Quantibody® Rat Array Slides
- Blocking Buffer
- Wash Buffer 1
- Wash Buffer 2
- Lyophilized Standard Mix
- Biotinylated Detection Antibody Cocktail
- Streptavidin-Conjugated Fluor
- Slide Washer/Dryer
- Adhesive Plastic Strips
- Manual
Other Materials Required
- Distilled or deionized water
- Small plastic boxes or containers
- Pipettors, pipette tips and other common lab consumables
- Orbital shaker or oscillating rocker
- Aluminum foil
- Gene microarray scanner or similar laser fluorescence scanner
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Learn MoreProtocol Outline
- Dry the glass slide
- Prepare Standards
- Block array surface
- Incubate with Samples and Standards
- Incubate with Biotinylated Detection Antibody Cocktail
- Incubate with Streptavidin-Conjugated Fluor
- Disassemble the glass slide
- Scan with a gene microarray laser scanner
- Perform densitometry and analysis
Storage/Stability
For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array slide(s), standard mix, detection antibody cocktail, and Cy3-Conjugated Streptavidin at -20°C and all other reagents undiluted at 4°C for no more than 3 months.