Rabbit Anti-Mouse DDIT3 (C-term A135) Antibody, 400 ul
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Product Description
Specifications
| Size | 400 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Mouse |
| Host Species | Rabbit |
| Accession Number | P35638 |
| Gene Id | 1649 |
| Gene Symbols | DDIT3 |
| Protein Name / Synonyms | DDIT3 (C-term A135) |
| Clonality | Polyclonal |
| Preparation | This DDIT3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 120-149 amino acids from the C-terminal region of human DDIT3. |
| Concentration (lot specific) | 0.500 mg/ml |
| Recommended Applications | FC, Western Blotting, IF |
| Research Area | Cancer, Autophagy |
| Shipping Type | Blue ice |
| Storage | -20°C |
Introduction
This gene encodes a member of the CCAAT/enhancer-binding protein (C/EBP) family of transcription factors. The protein functions as a dominant-negative inhibitor by forming heterodimers with other C/EBP members, such as C/EBP and LAP (liver activator protein), and preventing their DNA binding activity. The protein is implicated in adipogenesis and erythropoiesis, is activated by endoplasmic reticulum stress, and promotes apoptosis. Fusion of this gene and FUS on chromosome 16 or EWSR1 on chromosome 22 induced by translocation generates chimeric proteins in myxoid liposarcomas or Ewing sarcoma. Multiple alternatively spliced transcript variants encoding two isoforms with different length have been identified.
Specificity
Predicted Reactivity
Bovine, Hamster
Other Names
DDIT3; CHOP; CHOP10; GADD153; DNA damage-inducible transcript 3 protein; C/EBP-homologous protein; C/EBP-homologous protein 10; Growth arrest and DNA damage-inducible protein GADD153
NCBI Accession #
NP_001181982.1;NP_001181983.1;NP_001181984.1;NP_001181986.1;NP_004074.2
Other Accession #
P35639; P14607; Q0IIB6; NP_004074
Format
Type
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Calculated Molecular Weight (Da)
19175
Recommended Dilutions
IF: 1:10-50; WB: 1:1000; FC: 1:10-50
 | Fluorescent image of Hela cell stained with DDIT3 Antibody (C-term A135)(Cat# 102-11328/SA101207AA).Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with DDIT3 primary antibody (1:25, 1 h at 37°). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37°).Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37°). DDIT3 immunoreactivity is localized to Cytoplasm significantly. |
 | DDIT3 Antibody (C-term A135) (Cat# 102-11328) western blot analysis in mouse testis tissue lysates (35ug/lane).This demonstrates the DDIT3 antibody detected the DDIT3 protein (arrow). |
 | DDIT3 Antibody (C-term A135) (Cat# 102-11328) flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis. |
Antigen Source
HUMAN
Storage/Stability
2-8°C (short-term); -20°C (long-term)
Expiration:
12 months from the date of shipment when stored properly.