Description
Recombinant Dengue Multiple Epitopes 10 is genetically designed dengue multiple epitopes epi-10 designed especially for lateral flow product, they are selected from dengue genome. The rapid test prepared by this antigen has over 90% sensitivity and specificity of over 90% to show quick and strong signal for both dengue IgM and IgG. Conjugate gold suspension buffer, blocking reagent and running buffer den- epi-10 comes with the order.
Instructions for Use
- Prepare 526-532nm colloid gold, conjugation within 24 hours after colloid gold preparation
- Adjust colloid gold OD at 1.3-1.4, conjugation concentration and pH are 16 ug/ml gold and 7.2
- Shanking conjugation for 8-10 hours, then centrifuge the conjugation at 10,000 rpm at 20°C for 20 min
- Remove the supernatnant as more as possible, add the supplied gold suspension buffer to adjust OD to 8.2
- Add the supplied blocking reagent to the final concentration to 0.8%
- Blocking for 1 hour, then add sucrose to the final concentration to 10 to 12%
- Dipping the conjugated gold to the gold pad (do not use sodium phosphate for gold pad treatment)
- After drying, assemble gold pad, the sample pad and absorbent pad over backing card
- Add 10 ul serum/plasma and add the supplied epi-10 running buffer for assay
Stability & Storage
Storage/Stability
Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.