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Dengue Multiple Epitopes 13 Recombinant
Catalog #: 228-20485
Product Description
Specifications
| Size | 500 µg, 1000 µg |
| Estimated Lead Time | 2-3 weeks |
| Expression System | E. coli |
| Purity | Protein is >95% pure as determined by 12% PAGE (coomassie staining). |
| Formulation | Phosphate buffered saline, pH 7.4 and 0.02% sodium nitrate. |
| Research Area | Infectious Disease, Dengue |
| Shipping Type | Blue ice |
| Storage | -20°C |
Description
Description
Recombinant Dengue Multiple Epitopes 13 is genetically designed dengue multiple epitopes epi-13 designed especially for lateral flow product, they are selected from dengue genome. The rapid test prepared by this antigen has over 90% sensitivity and specificity over 90% to show quick and strong signal for both dengue IgM and IgG. Conjugate gold suspension buffer, blocking reagent and running buffer den- epi-13 comes with the order.
Instructions for Use
- Prepare 526-532nm colloid gold, conjugation within 24 hours after colloid gold preparation
- Adjust colloid gold OD at 1.3-1.4, conjugation concentration and pH are 16 ug/ml gold and 7.2
- Shanking conjugation for 8-10 hours, then centrifuge the conjugation at 10,000 rpm at 20°C for 20 min
- Remove the supernatnant as more as possible, add the supplied gold suspension buffer to adjust OD to 8.2
- Add the supplied blocking reagent to the final concentration to 0.8%
- Blocking for 1 hour, then add sucrose to the final concentration to 10 to 12%
- Dipping the conjugated gold to the gold pad (do not use sodium phosphate for gold pad treatment)
- After drying, assemble gold pad, the sample pad and absorbent pad over backing card
- Add 10 ul serum/plasma and add the supplied epi-10 running buffer for assay
Stability & Storage
Storage/Stability
Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.