Name: Denosumab ELISA Kit
SKU: EAD-Deno-1
Availability: InStock

Product Description

Specifications

Size	1 Plate Kit, 2 Plate Kit, 5 Plate Kit
Estimated Lead Time	3-5 business days
Sensitivity	0.021 ng/ml
Detection Range	0.021 ng/ml - 8 ng/ml
Specificity	This ELISA antibody pair detects Denosumab. Other targets not determined.
Compatible Sample Types	Cell Culture Supernatants, Plasma, Serum
Protein Name / Synonyms	Denosumab
Quantitative/Semi-Quantitative	Quantitative
Solid Support	96-well Microplate
Design Principle	Sandwich-based
Method Of Detection	Colorimetric
Research Area	Antibody Drug Development, Drug Antibody (DA) Detection
Shipping Type	Blue ice
Storage	≤-20°C

Application Notes

Kit Components

Pre-Coated 96-well Strip Microplate
Wash Buffer
Lyophilized Standard
Biotinylated Detection Antibody
Streptavidin-Conjugated HRP
TMB One-Step Substrate
Stop Solution
Assay Diluent

Other Materials Required

Microplate reader capable of measuring absorbance at 450nm
Precision pipettes to deliver 2 µl to 1 ml volumes
Adjustable 1-25 ml pipettes for reagent preparation
100 ml and 1 liter graduated cylinders
Absorbent paper
Distilled or deionized water
Log-log graph paper or computer and software for ELISA data analysis
Horizontal orbital microplate shaker
Tubes to prepare standard and sample dilutions

Protocol Outline

Prepare all reagents, samples and standards as instructed in the manual.
Add 100 µl of standard or sample to each well. Incubate 2 hours at room temperature.
Add 100 μl prepared biotinylated antibody to each well. Incubate 1 hour at room temperature.
Add 100 μl prepared Streptavidin solution. Incubate 45 minutes at room temperature.
Add 100 μl TMB One-Step Substrate Reagent to each well. Incubate 30 minutes at room temperature.
Add 50 µl of Stop Solution to each well. Read at 450 nm immediately.

Storage/Stability

The entire kit may be stored at -20°C for up to 6 months from the date of shipment. Avoid repeated freeze-thaw cycles.

Application Notes

Kit Components

Pre-Coated 96-well Strip Microplate
Wash Buffer
Lyophilized Standard
Biotinylated Detection Antibody
Streptavidin-Conjugated HRP
TMB One-Step Substrate
Stop Solution
Assay Diluent

Other Materials Required

Microplate reader capable of measuring absorbance at 450nm
Precision pipettes to deliver 2 µl to 1 ml volumes
Adjustable 1-25 ml pipettes for reagent preparation
100 ml and 1 liter graduated cylinders
Absorbent paper
Distilled or deionized water
Log-log graph paper or computer and software for ELISA data analysis
Horizontal orbital microplate shaker
Tubes to prepare standard and sample dilutions

Protocol Outline

Prepare all reagents, samples and standards as instructed in the manual.
Add 100 µl of standard or sample to each well. Incubate 2 hours at room temperature.
Add 100 μl prepared biotinylated antibody to each well. Incubate 1 hour at room temperature.
Add 100 μl prepared Streptavidin solution. Incubate 45 minutes at room temperature.
Add 100 μl TMB One-Step Substrate Reagent to each well. Incubate 30 minutes at room temperature.
Add 50 µl of Stop Solution to each well. Read at 450 nm immediately.

Storage/Stability

The entire kit may be stored at -20°C for up to 6 months from the date of shipment. Avoid repeated freeze-thaw cycles.

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