The immobilization of both single-stranded and double-stranded DNA onto microplate surfaces is a crucial step in many molecular biology and biochemistry assays, including those focused on DNA-protein interactions and DNA hybridization. Traditionally, this process has required expensive oligo modifications to facilitate DNA binding to plates, such as biotinylation for attachment to streptavidin-coated plates, or the use of activated ELISA plates for covalent binding of DNA. The RayBio^® Microplate DNA Coating Buffer offers a highly efficient and cost-effective alternative for binding unmodified DNA fragments as short as 50 base pairs to the surfaces of polystyrene 96-well microplates, as well as other polystyrene plastics.

Buffer should be stored at room temperature for a maximum of 12 months after delivery. Storage below room temperature can cause precipitate formation. If precipitate is observed heat solution to 37°C with gentle mixing until precipitate is dissolved.