25% off all ELISA kits

Use code ELISA25-262 for 25% off all ELISAs through November.

25% off all ELISA kits

Use code ELISA25-262 for 25% off all ELISAs through November.

E. coli LPS ELISA Kit

The RayBiotech Escherichia coli Lipopolysaccharide ELISA kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of process-related LPS, the common endotoxin impurities, derived from production processes when E. coli strains are utilized.

Lead time: Typically ships within 1-2 business days. No Friday shipments.

Product Description

Specifications

Size	1 Plate Kit, 2 Plate Kit, 5 Plate Kit
Estimated Lead Time	1-2 business days
Species	Escherichia spp.
Sensitivity	0.01 ng/ml Need more sensitivity? Then your answer is our Ultrasensitive Biomarker Testing Service powered by Simoa^™ technology.
Detection Range	0.01 ng/ml - 12 ng/ml
Specificity	This ELISA antibody pair detects E. coli LPS. The possibility of this antibody pair to detect LPS of other gram-negative bacteria has not been tested.
Compatible Sample Types	Cell Culture Supernatants, Cell Lysates
Quantitative/Semi-Quantitative	Quantitative
Solid Support	96-well Microplate
Design Principle	Sandwich-based
Method Of Detection	Colorimetric
Research Area	Bioprocesses
Shipping Type	Blue ice
Storage	-20°C

Product Features

Strip plates and additional reagents allow for use in multiple experiments
Quantitative protein detection
Establishes normal range
The best products for confirmation of antibody array data

Standard Curves

ELISA Kit Standard Curve Figures

Spiking & Recovery Results

Sample Type	Average % Recovery	Range (%)
Cell culture media	103.7	95-115
Cell lysis buffer	91.10	70-127
LB media	92.16	80-102

Linearity Results

Sample Type		Cell culture media	Cell lysis buffer	LB media
1:2	Average % of Expected	102.8	115.9	97.73
1:2	Range (%)	99-106	114-117	96-100
1:4	Average % of Expected	95.61	106.9	85.69
1:4	Range (%)	90-101	100-114	82-89

Application Notes

Kit Components

Pre-Coated 96-well Strip Microplate
Wash Buffer
Stop Solution
Assay Diluent(s)
Lyophilized Standard
Biotinylated Detection Antibody
Streptavidin-Conjugated HRP
TMB One-Step Substrate

Other Materials Required

Distilled or deionized water
Precision pipettes to deliver 2 µl to 1 µl volumes
Adjustable 1-25 µl pipettes for reagent preparation
100 µl and 1 liter graduated cylinders
Tubes to prepare standard and sample dilutions
Absorbent paper
Microplate reader capable of measuring absorbance at 450nm
Log-log graph paper or computer and software for ELISA data analysis

Protocol Outline

Prepare all reagents, samples and standards as instructed in the manual.
Add 100 µl of standard or sample to each well.
Incubate 2.5 h at RT or O/N at 4°C.
Add 100 µl of prepared biotin antibody to each well.
Incubate 1 h at RT.
Add 100 µl of prepared Streptavidin solution to each well.
Incubate 45 min at RT.
Add 100 µl of TMB One-Step Substrate Reagent to each well.
Incubate 30 min at RT.
Add 50 µl of Stop Solution to each well.
Read at 450 nm immediately.

Storage/Stability

The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Customize This E. coli LPS ELISA

We manufacture our ELISAs in-house, so we can build a custom version for your study.

Post-translational modifications (PTMs): Detect modified LPS (IRF6), including phosphorylation, acetylation, S-nitrosylation, S-acylation, oxidation, lactylation, sumoylation, ubiquitination, nitration, carbamylation, and more
Sample types: Validation in your matrix using samples you provide, such as CSF, urine, saliva, lysates, or exosomes
Also available: More sensitive platforms such as Simoa^® and NexaTag^®, multiplex panels, bulk lots, and other species when suitable reagents exist

Feasibility varies by target and modification, and we’ll tell you upfront what’s achievable.

Learn more about custom ELISA development

Have this product in hand?

Get your lot-specific datasheet at Ray's Docs. The datasheet linked below is our current version and may not match your lot.

Datasheet (ELW-LPS.pdf)

SDS (ELW-LPS.pdf)

Excel (Sandwich-ELISA-Analysis-Tool.xlsm)

Product Features

Strip plates and additional reagents allow for use in multiple experiments
Quantitative protein detection
Establishes normal range
The best products for confirmation of antibody array data

Standard Curves

ELISA Kit Standard Curve Figures

Spiking & Recovery Results

Sample Type	Average % Recovery	Range (%)
Cell culture media	103.7	95-115
Cell lysis buffer	91.10	70-127
LB media	92.16	80-102

Linearity Results

Sample Type		Cell culture media	Cell lysis buffer	LB media
1:2	Average % of Expected	102.8	115.9	97.73
1:2	Range (%)	99-106	114-117	96-100
1:4	Average % of Expected	95.61	106.9	85.69
1:4	Range (%)	90-101	100-114	82-89

Application Notes

Kit Components

Pre-Coated 96-well Strip Microplate
Wash Buffer
Stop Solution
Assay Diluent(s)
Lyophilized Standard
Biotinylated Detection Antibody
Streptavidin-Conjugated HRP
TMB One-Step Substrate

Other Materials Required

Distilled or deionized water
Precision pipettes to deliver 2 µl to 1 µl volumes
Adjustable 1-25 µl pipettes for reagent preparation
100 µl and 1 liter graduated cylinders
Tubes to prepare standard and sample dilutions
Absorbent paper
Microplate reader capable of measuring absorbance at 450nm
Log-log graph paper or computer and software for ELISA data analysis

Protocol Outline

Prepare all reagents, samples and standards as instructed in the manual.
Add 100 µl of standard or sample to each well.
Incubate 2.5 h at RT or O/N at 4°C.
Add 100 µl of prepared biotin antibody to each well.
Incubate 1 h at RT.
Add 100 µl of prepared Streptavidin solution to each well.
Incubate 45 min at RT.
Add 100 µl of TMB One-Step Substrate Reagent to each well.
Incubate 30 min at RT.
Add 50 µl of Stop Solution to each well.
Read at 450 nm immediately.

Storage/Stability

Customize This E. coli LPS ELISA

We manufacture our ELISAs in-house, so we can build a custom version for your study.

Post-translational modifications (PTMs): Detect modified LPS (IRF6), including phosphorylation, acetylation, S-nitrosylation, S-acylation, oxidation, lactylation, sumoylation, ubiquitination, nitration, carbamylation, and more
Sample types: Validation in your matrix using samples you provide, such as CSF, urine, saliva, lysates, or exosomes
Also available: More sensitive platforms such as Simoa^® and NexaTag^®, multiplex panels, bulk lots, and other species when suitable reagents exist

Feasibility varies by target and modification, and we’ll tell you upfront what’s achievable.

Learn more about custom ELISA development

LPS ELISA for ligand binding assay
Rating
The LPS ELISA kit was used for a ligand binding assay to assess interaction between lipids and LPS. The LPS used was from Sigma and hence, a standard curve was designed with the LPS ELISA to check detection of the coated antibody to the LPS used. The LPS ELISA did detect the Sigma LPS and the standard LPS provided with the kit in a similar manner and standard curve with both standards was comparable. Sample: Mixture of Lipid + LPS at different concentrations Standard ELISA concentrations: 2ng/mL, 4ng/mL, 5ng/mL, 6ng/mL 8ng/mL, 10ng/mL and 12ng/mL Experimental conditions: Same as the instruction provided in the kit. The LPS ELISA instructions were clear, easy to follow and can be used for ligand binding assays. However, the sensitivity of the ELISA falls at 2ng/mL and hence, should be used for detections at concentrations above 3ng/mL. As per the instructions manual, the substrate needs to be incubated for 30 min. However, an incubation between 15-20 min was sufficient for robust color determination.
Review by Gracy Rosario, from University of Missouri Kansas City, on Nov 13, 2025

Featured Products

25% off all ELISA kits

25% off all ELISA kits

E. coli LPS ELISA Kit