Rabbit Anti-Human EIF4E2 Antibody, 400 ul
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Product Description
Specifications
| Size | 400 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human |
| Host Species | Rabbit |
| Accession Number | O60573 |
| Gene Id | 9470 |
| Gene Symbols | EIF4E2 |
| Protein Name / Synonyms | EIF4E2 |
| Clonality | Polyclonal |
| Preparation | This EIF4E2 antibody is generated from rabbits immunized with human EIF4E2 recombinant protein. |
| Concentration (lot specific) | 0.5 mg/ml |
| Recommended Applications | IHC-P, Western Blotting |
| Shipping Type | Blue ice |
| Storage | -20°C |
Introduction
EIF4E2 is expressed exclusively in the cytoplasm. This protein recognizes and binds the 7 methylguanosine containing mRNA cap during an early step in the initiation of protein synthesis and facilitates ribosome binding by inducing the unwinding of the mRNAs secondary structures.
Specificity
Other Names
EIF4E2; EIF4EL3; Eukaryotic translation initiation factor 4E type 2; Eukaryotic translation initiation factor 4E homologous protein; Eukaryotic translation initiation factor 4E-like 3; eIF4E-like protein 4E-LP; mRNA cap-binding protein 4EHP; mRNA cap-binding protein type 3
NCBI Accession #
NP_004837.1
Format
Type
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Calculated Molecular Weight (Da)
28362
Recommended Dilutions
WB: 1:1000; IHC: 1:10-50
 | Western blot analysis of EIF4E2 Antibody (Cat# 102-14324) in NCI-H460 cell line lysates (35ug/lane). EIF4E2 (arrow) was detected using the purified Pab. |
 | Western blot analysis of EIF4E2 (arrow) using rabbit polyclonal EIF4E2 Antibody (Cat# 102-14324). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the EIF4E2 gene. |
 | Formalin-fixed and paraffin-embedded human breast carcinoma reacted with EIF4E2 Antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. |
Antigen Source
HUMAN
Storage/Stability
2-8°C (short-term); -20°C (long-term)
Expiration:
12 months from the date of shipment when stored properly.