ELISA Services

On each 96 well plate, an 8-point standard curve is run with duplicate wells. The remaining wells may then be used for samples (duplicates are strongly recommended). If the entire plate is used in 1 experiment, the remaining 80 wells will accommodate 40 samples in duplicate. If not run all at once, a new standard curve must be included with every run, reducing the number of potential samples by 8 (to account for the new standard curve points).

The ELISAs with a longer, typically 4 - 7 week, lead times contain antibody pairs and standards that have been validated for multiplex array products, but not for the 96-well plate ELISA format. As a result, they require a development phase that RayBiotech begins after receiving an order for the kit. After development, the kit will have passed RayBiotech's ELISA quality control tests and will, thereafter, have a typical 1 - 2 day lead time for orders placed.

Our sandwich ELISA kits are evaluated for intra-assay reproducibility and precision by running 2-3 positive control samples in duplicate on a single plate. Inter-assay reproducibility is evaluated by at least 2 independent experiments with 2-3 positive control samples and a full standard curve. Lot-to-lot consistency is tested by comparing calculated concentrations of 2-3 positive control samples with calibration curves of current lot and previous lot.

Recovery is determined by spiking various levels of target protein into biological samples. Dilution linearity is tested by performing 2-fold and 4-fold dilutions of biological samples. Recoveries typically range from 80-130%.

The ELISA kits with catalog numbers ending in “-CL” are optimized and validated for use with cell and tissue lysate samples. All other ELISA and EIA kits (catalog numbers without “-CL”) have not been validated by RayBiotech with cell or tissue lysates, however RayBio kits can generally accommodate many different samples types. All RayBio Phosphorylation ELISA kits are validated with cell lysates.

Start by preparing your sample(s) - Tips on Sample Preparation. Then, place each sample into a labeled vial. Freeze the sample right after collection, if possible. You can use a quick freeze procedure. Include a printed copy of the sample service form with your shipment and indicate the quote number or what type of service you’ve requested (C-Series, Quantibody, ELISA, etc.) on the shipping label. Samples must be free of infections, such as HIV, Hepatitis B, or other communicable viruses, etc.

View more detailed instructions here.