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Eotaxin, Mouse Recombinant (CCL11)
Catalog #: 228-10397
Product Description
Specifications
| Size | 20 µg, 1000 µg |
| Estimated Lead Time | 2-3 weeks |
| Species | Mouse |
| Accession Number | P48298 |
| Gene Id | 20292 |
| Protein Name / Synonyms | Small inducible cytokine A11, CCL11, Eosinophil chemotactic protein, chemokine (C-C motif) ligand 11, SCYA11. |
| Expressed Region | The sequence of the first five N-terminal amino acids was determined and was found to be His-Pro-Gly-Ser-Ile. |
| Expression System | E. coli |
| Purity | Greater than 95.0% as determined by:(a) Analysis by RP-HPLC.(b) Analysis by SDS-PAGE. |
| Format | Sterile Filtered White lyophilized (freeze-dried) powder. |
| Formulation | Lyophilized from a concentrated (1mg/ml) solution in water containing no additives. |
| Activity | The biological activity was determined by measuring the dose dependent phosphorylation of ERK1 and ERK2 in CCR3 transfected 293 cells. Significant ERK phosphorylation is observed with 100 ng/ml of recombinant mouse Eotaxin. |
| Reconstitution | It is recommended to reconstitute the lyophilized Eotaxin in sterile 18MΩ-cm H20 not less than 100µg/ml, which can then be further diluted to other aqueous solutions. |
| Research Area | Angiogenesis, Inflammation, Wnt-beta-Catenin Signaling, Chemokine Biology |
| Shipping Type | Ambient temperature |
| Storage | -20°C |
Description
Description
Eotaxin Mouse Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 74 amino acids and having a molecular mass of 8403.2 Dalton. The CCL11 is purified by proprietary chromatographic techniques.
Stability & Storage
Storage/Stability
Lyophilized Eotaxin although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution CCL11 should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles.