Etanercept (Enbrel®) immunoassay kit enabling sensitive quantification of drug concentrations across serum, plasma, and cell culture supernatants.
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Product Description
Specifications
| Size | 1 Plate Kit, 2 Plate Kit, 5 Plate Kit |
| Estimated Lead Time | 3-5 business days |
| Species | Human |
| Sensitivity | 17 pg/mL |
| Detection Range | 17 pg/mL - 8.0 ng/mL |
| Specificity | This ELISA antibody pair detects Etanercept. Other targets not determined. |
| Compatible Sample Types | Cell Culture Supernatants, Plasma, Serum |
| Recommended Dilution (Serum/Plasma) | 300-fold |
| Accession Number | DB00005, P20333 |
| Protein Name / Synonyms | Etanercept, etanercept-szzs, etanercept-ykro, Recombinant human TNF, rhu TNFR:Fc, rhu-TNFR:Fc, TNFR-Immunoadhesin |
| Quantitative/Semi-Quantitative | Quantitative |
| Solid Support | 96-well Microplate |
| Design Principle | Sandwich-based |
| Method Of Detection | Colorimetric |
| Research Area | Antibody Drug Development, Drug Antibody (DA) Detection |
| Shipping Type | Blue ice |
| Storage | ≤-20°C |
Standard Curve
Kit Components
- Pre-Coated 96-well Strip Microplate
- Lyophilized Standard
- Detection Antibody
- HRP-Streptavidin
- Wash Buffer
- Assay Diluent
- TMB One-Step Substrate Reagent
- Stop Solution
Additional Materials Required
- Microplate reader capable of measuring absorbance at 450 nm
- Protease and Phosphatase inhibitors
- Precision pipettes to deliver 2 μL to 1 mL volumes
- Adjustable 1-25 mL pipettes for reagent preparation
- 100 mL and 1 liter graduated cylinders
- Log-log graph paper or computer and software for ELISA data analysis
- Absorbent paper
- Tubes to prepare standard or sample dilutions
- Distilled or deionized water
- Shaker
Assay Procedure Summary
- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 µL of each standard and sample to each well. Incubate 2 hours at room temperature or overnight at 4ºC with gentle shaking.
- Add 100 μL prepared detection antibody to each well. Incubate for 1 hour at room temperature with gentle shaking.
- Add 100 μL prepared HRP-Streptavidin solution. Incubate for 45 minutes at room temperature with gentle shaking.
- Add 100 μL TMB One-Step Substrate Reagent to each well. Incubate 30 minutes at room temperature.
- Add 50 μL Stop Solution to each well. Read at 450 nm immediately.
Storage/Stability
The entire kit may be stored at -20°C for up to 6 months from the date of shipment. Avoid repeated freeze-thaw cycles.
