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ISO 13485:2016 cGMP

Fibroblast Growth Factor-Basic, Human Recombinant

Catalog #: 228-10452

Product Description

Specifications

Size50 µg, 1000 µg
Estimated Lead Time2-3 weeks
SpeciesHuman
Accession Number
P09038
Gene Id
2247
Protein Name / Synonyms
Prostatropin, HBGH-2, HBGF-2, FGF-2, FGF-b.
Expressed Region
AAGSITTLPA LPEDGGSGAF PPGHFKDPKR LYCKNGGFFL RIHPDGRVDG VREKSDPHIK LQLQAEERGV VSIKGVCANR YLAMKEDGRL LASKCVTDEC FFFERLESNN YNTYRSRKYT SWYVALKRTG QYKLGSKTGP GQKAILFLPM SAKS.
Expression SystemE. coli
Purity
Greater than 98.0% as determined by analysis by SDS-PAGE.
Format
Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation
The protein was lyophilized from a 0.2µm filtered concentrated solution in 20nM Tris-HCl, pH7.4 and 1M NaCl.
Activity
The ED50, calculated by the dose-dependant proliferation of murine balb/c 3T3 cells is <0.1ng/ml, corresponding to a specific activity of greater than 1.0x107 Units/mg.
Research AreaAngiogenesis
Shipping TypeAmbient temperature
Storage-20°C

Description

Description

Fibroblast Growth Factor-2 Human Recombinant (FGF-2) produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 154 amino acids and having a molecular mass of 17.2kDa.The FGF-b is purified by proprietary chromatographic techniques.

Stability & Storage

Storage/Stability
Lyophilized Fibroblast Growth Factor-2 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution FGF-b should be stored at 4°C between 2-7 days and for future use below -18°C.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles.